目的:探讨甲状腺功能低下的早产儿血清游离三碘甲状腺原氨酸(Free trilute,FT3)、游离甲状腺激素(Free thyroxin,FT4)、促甲状腺激素(Thyroid stimulation hormone,TSH)水平进行动态态变化及临床意义。方法:我院在2007年11月至2008年4月住院的32例诊断为甲状腺功能低下的早产儿为研究对象,应用放射免疫法检测生后第1天,第7天,第15天,1月和2月血清FT3、FT4、TSH水平。结果:32例诊断为甲状腺功能低下的早产儿中,有窒息史的22例,呼吸窘迫综合症(Respiratory distress syndrome,RDS)10例,单纯性早产5例;出现少吃少动临床症状6例;生后各时期的TSH变化没有统计学差异,生后1、5天与生后1、2月FT3、FT4则可见Plt;0.05具有统计学差异。结论:有窒息和RDS的早产儿中,容易发生甲状腺功能降低,以低甲状腺素血症最为常见,使用左旋甲状腺素后,FT-3、FT-4水平可迅速恢复正常。如果同时存在TSH增高,则TSH水平恢复较慢。
摘要:目的:探讨卡配因抑制剂3（MDL28170）对新生大鼠缺氧缺血性脑损伤(HIBD)神经细胞凋亡的影响。方法:建立新生SD大鼠HIBD模型,治疗组于缺养缺血后即刻、2 h、4 h腹腔内注射MDL28170,对照组及手术组同时予生理盐水。缺氧缺血后24 h用免疫组化方法观察大脑皮质及海马CA1区Caspase3 蛋白表达、TUNEL法检测细胞凋亡,观察组织病理改变并计算海马神经元死亡数,透射电镜观察细胞超微结构。结果:缺氧缺血后24 h缺血侧大脑皮质及海马CA1区Caspase3和TUNEL阳性细胞数较对照组明显增加,透射电镜证实有凋亡细胞;MDL28170可减少阳性细胞数量,抑制神经元死亡,差异有显著性(Plt;0.05)。结论:MDL28170可通过抑制神经凋亡而对新生大鼠HIBD具有一定保护作用。Abstract: Objective: To investigate the effect of (Calpain inhibitor3) MDL28170 on neural apoptosis in a neonatal model of hypoxicischemic brain damage (HIBD). Methods: A neonatal model of HIBD was established, 7dayold SD rats were divided into three groups. The treatment group received MDL28170(ip) at 0 h,2 h,4 h after HI, whereas the other two groups were administered normal saline simultaneously. The expression of caspase3 (by immunohistochemistry), neural apoptosis (by TUNEL) in cortex and hippocampus ipsilateral to the insult were observed 24 h after HI; hippocampal CA1 neural loss and electromicroscopic changes were assessed at the same time. Results: Apoptotic body was observed by electromicroscopy. Caspase3 positive cells and apoptotic cells increased significantly in the ipsilateral cortex and hippocampal CA1 region compared to the control, and MDL28170 reduced the number of positive cells, attenuated CA1 neural loss with significance (Plt;0.05). Conclusion: It is suggested that MDL28170 may protect the brain of neonatal rats after HIBD by suppressing neural apoptosis.