ObjectiveTo explore the effects of burn ward cleaning methods on multi-drug resistant bacteria infection, in order to improve and optimize the cleaning process and method. MethodsFrom November 2012 to October 2013, the cleaning and disinfection methods in our burn wards were regarded as the traditional cleaning methods, and from November 2013 to October 2014, the cleaning and disinfection methods were called the improved cleaning methods (new system cleaning methods). By retrospective analysis, we compared the infection rates of multi-drug resistant bacteria before and after the implementation of the new system cleaning methods. ResultsNew system methods were used in the ward environment cleaning and disinfection. The infection rate of multi-drug resistant bacteria before and after the implementation of the new system cleaning methods were 12.414‰ and 5.922‰ respectively. The methicillin resistant Staphylococcus aureus infection rate was 7.286‰ and 3.718‰, and the carbon-resistant Pseudomonas aeruginosa infection rate was 2.699‰ and 0.689‰. Both differences were significant (P < 0.05). The carbon-resistant Acinetobacter baumanii infection rate was 2.429‰ and 1.515‰ before and after the implementation of the new methods with no significant difference (P > 0.05). ConclusionAdopting new system to carry out cleaning can effectively reduce the infection rate of multi-drug resistant bacteria in the burn ward, and it is worthy of clinical popularization and application.