Objective To constitute a new collagen gel artificial skin by using ch ito san as one of the components. Methods Human fo resk in fibroblasts were incorporated into thechitosan-collagen-GAGs to constitute dermal equivalent(DE). The growth of fibroblasts incorporated in gels and several factors which influenced the contraction of the gel were observed. The influence of different chitosan contents on the growth of fibroblast and keratinocyte and on the antibacterial effect were studied. Keratinocytes separated from normal children foresk in were seeded on the matured DE to reconstruct artificial skin, which was immersed at the early stage of culture, then lifted to an air-liquid interface. The structure of the DE and artificial skin were analysed by histology and scanning electron microscope. Results The contraction rate of the DE was proportional to the number of fibroblasts, and the final size of the DE was inversely proportional to the concent ration of collagen protein. Fibroblasts incorporated into the gel showed the exponential growth from the 2nd day to the 9th day. Chitosan-collagen-GAGs had no inhibition effect on the growth of fibroblasts, but promoted the growth of eratinocytes. Staphylococcus aureus was inh ibited even more as chitosan content increased. Scanning electron micro scopy indicated that the DE had abundant porous fabrication. Artificial skin shared some histological features of normal skin, which consisted of a good strat ifiedepiderm is and a dense dermis. Conclusion Chitosan-Collagen-GAGs collagen gelart ificial skin is a new collagen gel living artificial skin which has certain antibacterial ability and stratified epiderm is and dense dermis structure like normal skin.