Objective To determine the ability of the polyactic acid glue (PLA-G) in preventing epidural adhesion after laminectomy. Methods Each of the 24 randomly selected rats was done laminectomyof L2 and L5. The PLA-G, which would change from liquid to solid when meets with serum, was used in the epidural site of L5 to become a half-solid membrane(the experimental group, EG). The PLA-G was not used in the same site of L2(the control group, CG) .The gross anatomical, histological, and microscopical evaluation were made 2，4，6，8，10 and 12 weeks after operation; electron microscope observation was also made on two rats 4 weeks after operation(both EG and CG). Results Obvious epidural space was observed between dura mater and scar tissue(ST), but no cells in the epidural space were observed inEG 2 weeks after operation. Corps of the red cells between dura mater and ST and proliferation of fibroblast cell(FB) were observed in CG 2 weeks after operation. Some remaining glue shiver in the epidural space with lightly increasing fibroblast and smooth dura mater were observed in EG 4 weeks after operation. However, fragile scar conglutinated with dura mater diffusely and more FB were observed in CG. From the 6th week to the 12th week, a potential interspace between scar and dura mater was observed and PLA glue was absorbed completely in EG. However, tougher scar, which was very difficult to dissect from dura mater and surrounding tissues, was observed. According to the fibroblast ultrastructure observation, bigger nucleus and more plentiful rough endoplasmic reticulum were observed in CG. Conclusion The PLA-G can effectively reduce the epidural scar and adhesion in animal experiment.