ObjectiveTo evaluate the clinical efficacy of fish oil containing lipid emulsion (FO) in sepsis. MethodsRandomized controlled trials about fish oil containing lipid emulsion in sepsis,which were published from 1980,were searched from the following electronic databases:PubMed,Embase,Foreign Medical Journal Service,Cochrane Library,CNKI,Wanfang Database,and VIP Database. The articles screening,quality assessment and data extraction were conducted by two reviewers independently. The methodological quality of trials was assessed by Jadad's scale. All data was analyzed by Review Manager 5.2 software. ResultsFifteen studies involving 794 participants met the inclusion criteria. The meta-analysis results were as follows:compared with the control group,①FO could shorten the length of ICU stay[WMD=-5.59,95%CI(-7.65,-3.53)] and the length of hospital stay[WMD=-10.48,95%CI(-14.67,-6.29)],and also decrease the 28-day mortality[RR=0.69,95%CI(0.52,0.93)]. ②FO could reduce the concentration of TNF-α[WMD=31.78,95%CI(5.63,57.92)] and improve oxygenation index[WMD=41.95,95%CI(30.80,53.11)] in septic patients. ③There was no statistical significance in the decrease of C-reactive protein (CRP) between two groups(P>0.05). ConclusionThe administration of FO in septic patients can significantly shorten the length of ICU stay and hospital stay,decrease the 28-day mortality,reduce the concentration of TNF-α and improve oxygenation index. However,it shows no significant difference in the decrease of CRP compared with the administration of traditional lipid emulsion. All of the above results show that the septic patients can benefit from the administration of fish oil containing lipid emulsion.
Objective To assess the safety of intravenous infusion with self-made perfluorocarbon emulsion (PFE) in rats.Methods 35 Wistar rats were randomly divided into a normal control group and six PFE groups (n=5 in each group).The control group was intravenous injected with normal saline and six PFE groups with PFE.Blood samples were obtained from the PFE groups 5 min after injection via vena orbitalis for perfluorocarbon (PFC) measurement.The control group were sacrificed at 2 hours after injection，and the six PFE groups were respectively killed at 2 h，4 h，6 h，24 h，48 h and 10 d after injection.The blood levels of PFC，PaO2，GPT，GOT，BUN and Cr were measured.Pathological changes of lung，liver and kidney were also observed.Results All rats were in good health state after PFE intravenous injection with no obvious pathological changes in liver and kidney.PFC was wide distributed throughout the pulmonary alveoli and capillaries.The levels of GPT and GOT were higher in the PFC groups at 2，4，6 and 24 h than which in the control group (all Plt;0.05)，but there were no significant difference between the PFE 10 d group and the control group.The levels of BUN and Cr had no remarkably differences in all groups.Blood PFC concentrations were (20±1.8)mg/mL，(1.8±0.7)mg/mL，(1.5±0.6)mg/mL，(1.2±0.4)mg/mL，(0.5±0.2)mg/mL，(0.2±0.03)mg/mL，0 mg/mL in the PFE groups respectively at 5 min，2 h，4 h，6 h，24 h，48 h，10 d after injection.PaO2 of the PFE 2 h group was significant higher than which in the control group [(119.2±8.6)mm Hg vs (99.6±4.7)mm Hg，Plt;0.05].Conclusion Intravenous injection with PFE (10 mL/kg) can enhance PaO2 with good safety and no obvious influence on pathology of lung，liver and kidney，so may be used for the treatment study of acute lung injury.
OBJECTIVE: To explore a new method of preparing the composite of DL-polylactic acid (PDLLA), hydroxyapatite(HA), decalcium bone matrix (DBM), and to observe the degradation characteristics of PDLLA/HA/DBM in vitro. METHODS: An emulsion blend method was developed to prepare the composite of PDLLA/HA/DBM based on the weight rate of PDLLA:HA:DBM = 1.5-2:1-1.5:1. The characteristics of the particles was observed by scanning electron microscope. In vitro, PDLLA/HA/DBM and PDLLA were put into PBS(pH7.4) respectively; the pH value, weight and biomechanics of them were determined during the degradation. RESULTS: Without heating, the emulsion blend method could be developed to prepare PDLLA/HA/DBM. Scanning electron microscope showed that the gap diameter in the compound material was 100 to 400 microns, and the porosity was 71.3%; During degradation, the pH value of PDLLA decreased little within 2 weeks, then decreased obviously and decreased to 4.0 at the end of the 4th week; while the pH value of PDLLA/HA/DBM kept quite steady and was 6.4 at the end of the 12th week. The weight of PDLLA decreased little within 4 weeks, then decreased obviously and remained 50% of its prime weight at the end of the 12th week; while the weight of PDLLA/HA/DBM decreased little within 5 weeks, then decreased obviously and remained 60% of the prime at the end of the 12th week. The prime biomechanical strength was 1.33 MPa in PDLLA and 1.71 MPa in PDLLA/HA/DBM. There was significant difference between them (P lt; 0.05). The strength of PDLLA decreased little within 3 weeks, then decrease obviously and was 0.11 MPa at the end of the 12th week; the strength of PDLLA/HA/DBM decreased little within 4 weeks, then decrease obviously and was 0.21 MPa at the end of the 12th week. CONCLUSION: The emulsion blend method is a new method to prepare bone repair materials. As a new bone repair material, PDLLA/HA/DBM is suitable for bone tissue engineering for its good characteristics of porosity and degeneration.