目的：分析体外受精(IVF)患者卵泡液中sFas和sFasL的浓度与妊娠的关系，以及与卵泡液中的促黄体素(LH)水平之间的相关性。方法：收集行IVF/ICSI患者的卵泡液，测定sFas、sFasL和LH浓度。结果：妊娠组排卵前卵泡液中的sFasL和LH水平显著高于未妊娠组(P分别为0.002和＜0.001)，两组中sFas水平没有明显差异。通过logistic回归分析，卵泡液中与妊娠有统计学相关的因素仅为LH，OR=4.117(P为0.001)。卵泡液中的LH和sFasL的水平呈明显正相关关系(Plt;0.001)，LH与sFas之间无显著相关关系。结论：在IVF/ICSI治疗周期中，卵泡液中的sFasL蛋白和LH水平与妊娠结局有关，卵泡液中的LH水平可能通过调节卵泡局部的某些系统来调节卵泡中FasFasL系统介导的凋亡作用。
ObjectiveTo evaluate the effect of pre-infusion of allogeneic lymphoyctes treated with 5-FU on the rat liver graft. MethodsRat liver transplant models from Wistar to SD were established. Four groups were designed as following: control group: only liver transplantation without any other intervention; lymphocytes group: 1 ml of untreated lymphocytes (5×106/ml) from Wistar rats were preinfused into SD rats on day 7 and 4 separately before transplantation; lymphocytes with low concentration of 5-FU group: low concentration 5-FU (7.5 μg) treated lymphocytes were preinfused as above; lymphocytes with high concentration of 5-FU group: high concentration 5-FU (15 μg) treated lymphocytes were preinfused as above. Fas-L and CD8 expression were detected by immunohistochemistry method on day 7 after transplantation. ResultsThe integral opticaldensity (IOD) of Fas-L positive lymphocytes in the lobules of liver and portal areas were higher in lymphocytes with low concentration of 5-FU group than in the other groups （Plt;0.05）. There was no difference between lymphocyte group and lymphocytes with high concentration of 5-FU group （Pgt;0.05）. The IOD of CD8+ expression in lobules of liver was not different among all the three lymphocytes treated groups （Pgt;0.05）. But in portal areas, CD8+ expression was lower in the lymphocytes with low concentration of 5-FU group than in the other groups （Plt;0.05）. ConclusionPreinfusion of lymphocytes treated with low concentration 5-FU can induce graft immune tolerance, the probable mecanism of which is the increasing Fas-L expression in graft.
Objective To investigate the rationale of immune privilege of testicular sertoli cell. Methods Testicular sertoli cell was prepared by digested collagenase, trypsin, and Dnase. In vitro, the sertoli cells were culture together with active lymphocytes to observe the effect on killing lymphocytes. SABC was used for labeling the Fas ligand on testicular sertoli cell.Results In vitro, sertoli cell can kill the active lymphocytes, and testicular sertoli cell expresses the Fas ligand. Conclusion Fas ligand expressing on the testicular sertoli cell may be the cause of immune privilege of testicular.