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find Keyword "Gene expression/genetics" 1 results
  • Expression of opticin in the eyes of diabetic mice

    Objective To observe the opticin expression in the eyes of nonobese diabetes (NOD) mice and nondiabetic NOD mice.Methods Twenty NOD mice were divided into diabetic group (experimental group) and nondiabetic group (control group). All the mice were killed by cervical dislocation method.The eyes were harvested, and the vitreous, retina and sclera were separately collected. Western blot and realtime reverse transcriptionpolymerase chain reaction(RT-PCR)were respectively used to determine opticin protein and OPTC mRNA levels.Results The opticin protein level in the vitreous and retina was lower in the experimental group(t=4.42,4.58;P=0.002,0.002),but is same in thesclera between the 2 groups(t=0.27,P=0.794).OPTCmRNA level was vitreousgt;retinagt;sclera. OPTCmRNA levels of vitreous and retina in diabetic group were significantly lower(t=3.30,2.48;P=0.01,0.04); there was no statistical significant on OPTC mRNA of sclera between two groups(t=0.27,P=0.80).Conclusion Expression of opticin was suppressed in retina and vitreous of diabetic mice.

    Release date:2016-09-02 05:40 Export PDF Favorites Scan
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