【Abstract】Objective To investigate the injury of calcium on the liver. Methods By using collagenase-containing solution to perfuse the rat livers, the rat liver suspension with different viability was prepared, preserved hypothermically, and the cytosolic calcium comcentration was detected with Fura2/AM. Results ① The concentration of cytosolic calcium after 2-hour storage: Experiment group 1(viability 5%) (1055.0±30.79) nmol/L, experiment group 2(viability 10%) (853.0±20.42) nmol/L, experiment group 3(viability 30%) (616.0±13.20) nmol/L, experiment group 4(viability 50%) (562.0 ±26.06) nmol/L, experiment group 5(viability 70%-80%) (318.0±13.01) nmol/L, experiment group 6(viability 90%) (114.6±6.11) nmol/L. ②The concentration of cytosolic calcium after 24hour storage: Experiment group A(viability 10%) (1704.0±70.11) nmol/L, experiment group B(viability 50%) (1125.0±23.22) nmol/L. The results showed that the lower was the viability, the higher was the cytosolic calcium concentration.With the same viability the cytosolic calcium concentration elevated more than two times higher than the original concentration with the time lengthened. Conclusion Calcium overload is one of the main factors which attribute to the ischemiareperfusion injury of the hepatocytes.
【Abstract】Objective To investigate the effects of preservative temperature on pancreatic function and determine the optimal preservative temperature for pancreatic transplantation. MethodsCold pancreatic preservation was performed and a homologous male Wistar rat model of heterotopic total pancreaticoduodenal transplantation was established. The pancreas was preserved for 6 h in UW solution at specific temperatures(0 ℃, 4 ℃, 8 ℃ and 12 ℃). After preservation, pancreatic tissue was taken for histologic examination in every group. ATP and total adenine nucleotides (TAN) were determined by using high performance liquid chromatography (HPLC). Blood glucose(BG), serum amylase and lipase were measured 24 h after transplantation. And the activity of myeloperoxidase (MPO) in the pancreatic grafts was also measured at the same time. Besides, histological observation was performed. Results Microscopic studies showed that the histomorphological changes of pancreas in 4 ℃ group were less obvious than those in other groups. Tissual concentrations of ATP and TAN decreased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group after 6 h of preservation(PH＜0.05). The levels of BG, serum lipase and MPO increased gradually in 4 ℃ group, 0 ℃ group, 8 ℃ group, and 12 ℃ group(PH＜0.05). The activity of MPO in 4 ℃ group （1.19±0.16 U/g ）was significantly lower than that of the control group（0.26±0.09 U/g，PH＜0.05). Conclusion The temperature of 4 ℃ is most appropriate for hypothermic pancreatic preservation and can considerably alleviate cold ischemic injury of rat pancreas.