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"HE Xiangge" 2 results
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Objective To observe the content of thromboxane (TXA2 ) and prostacyclin (PGI2) in optic nerves after forehead impact injury.Methods The right forehead zones of 32 rabbits were struck by biology impact machine. Tweenty-four rabbits that had afferent papillary defect after injury were chosen, and randomly divided into four groups: 1 day, 2, 4, and 7 days group. Right eyes were in the experimental group and left eyes were in the control group. Flash visual evoked potentials were examined before and after the traumatic injury. The rabbits ′eyes were removed, the optic nerves were pathologically examined, and the content of TXB2 and 6-Keto-PGF1αwhich were the products of TXA2 and PGI2 were assayed 1, 2, 4, and 7 days after traumatic injury respectively.Results Histopath ological examination revealed the findings of injuries of optic nerves of all the 24 rabbits. The latency of wave P1 was significantly delayed after traum atic injury (Plt;0.01), and amplitude of wave P1 was significantly decreased after traumatic injury (Plt;0.01). The content of TXB2 ［(172.35±26.52) pg/mg ］and 6-Keto-PGF1α［(161.78±24.83) pg/mg］were significantly higher in the injured optic nerves than in the uninjured ones 1 day after the traumatic injury (Plt;0.01). The rate of TXB2 /6-Keto-PGF1α (1.077±0.18) was significantly increased compared to the control group (Plt;0.05), and lasted to the 7th day.Conclusions The content of TXA2 and PGI2 significantly increases and the ratio of them is lopsided after forehead impact injury in rabbits. (Chin J Ocul Fundus Dis，2003，19：49-51)
Release date：2016-09-02 06:00
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Objective
To observe the effect of ciliary neurotrophic factor (CNTF) with different concentrations on the growth and survival of ratsrsquo; retinal ganglion cells (RGC) in vitro.
Methods
The retinae of 15 Wistar rats which were 2 or 3 days after birth were dissociated into cell suspension with 0.05% trypsin digestion. After 3 days, cultured RGC were identified with immunohistochemistry method using anti-rat Thy-1.1 monoclonal antibody. Cultured RGC were divided into the 10, 20, 40 ng/ml CNTF group （Ⅰ,Ⅱ, and Ⅲgroup） and the control group respectively. The duration of living RGC was recorded. After 3, 5 and 7 days, the A value of living cells was tested by methylthio-tetrazole colorimetric microassay.
Results
The result of immunohistochemical examination showed that 90% of living cells cultured for 3 days were RGC. No protuberance or volume increase of RGC were observed in CNTF groups and the control group. The duration of the living RGC was prolonged 3 to 4 days in CNTF groups compared with the control group. The A values of living RGC at the 5th and 7th days in the CNTF groups and the control group were: 0.0758plusmn;0.0139 and 0.0693plusmn;0.0113 in I group, 0.0902plusmn;0.0114 and 0.0825plusmn;0.0125 in Ⅱ group, 0.0792plusmn;0.0133 and 0.0653plusmn;0.0086 in Ⅲ group, and 0.0620plusmn;0.0071 and 0.0513plusmn;0.0068 in the control group, respectively. The differences between the simultaneous CNTF and control group were significant (between Ⅱ group and the control group: P＜0.01; between Ⅰ and Ⅲ group, and the control group: P＜0.05).
Conclusion
CNTF with some certain concentrations could facilitate survival of RGC in vitro. CNTF has no effect on the conformation of RGC.
(Chin J Ocul Fundus Dis, 2002, 18: 283-285)
Release date：2016-09-02 06:01
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