Objective To investigate the comfort status of patients undergoing video electroencephalogram (VEEG) examination and explore its influencing factors. Method A total of 177 patients who underwent VEEG examination in the Second Affiliated Hospital of Guangzhou Medical University from March 2023 to February 2024 were selected as the research subjects, and their comfort status and influencing factors during the examination period were analyzed. Result The comfort level of patients undergoing VEEG examination was at a moderate to high level (68.9% and 31.1%, respectively); complete mastery of VEEG health knowledge accounted for 49.2% and 65.5% in the moderate and high comfort groups, respectively. Logistic regression analysis results showed that head discomfort and lack of knowledge of VEEG health were independent influencing factors on the comfort of VEEG examination (P<0.05). Conclusions The comfort level of patients undergoing VEEG examination is at a moderate to high level of comfort; the overall mastery of knowledge related to video electroencephalography is insufficient and still needs further improvement; medical staff should pay attention to the comfort of patients and their mastery of relevant knowledge in examinations. They should take targeted intervention measures in a timely manner based on influencing factors, improve their knowledge level and cooperation with VEEG examinations, reduce the occurrence of discomfort during examinations, and improve the overall comfort level of VEEG examinations.