Objective To investigate the effects of activities of Na+K+ATPase in plasma membranes of hepatocytes on the formation of calcium bilirubinate gallstone. MethodsThe research were studied in Xiao’s rabbit models.One hundred and three rabbits were randomly divided into the control group (Con,n=28),simple biliary obstruction group (BO,n=36),biliary obstruction and infection group (BOI,n=39).The activities of Na+K+ATPase,contents of intracellular calcium and bile acids in bile were measured in 3rd,7th,14th and 20th day. ResultsThe activities of Na+K+ATPase,contents of bile acids in bile decreased gradually and the contents of intracelluar calcium of hepatocytes increased continously in all phases of experimental groups compared with control group (P＜0.01).There was more significant changes in BOI group than in BO group (P＜0.05). Conclusion The progressive decrease of activities of Na+K+ATPase has a close relationship with the overloaded intracellular calcium and the continous decrease of bile acids contents in bile in formation of calcium bilirubinate gallstone.Infection can promote above mentioned changes and more stone formation.
From this experiment we found that acetylcholine (Ach) was the agonist of the colonic smooth muscles, and noradrenaline (NA) the internal anal sphincter(IAS). The contractions of colonic smooth muscles were significantly inhibited under the calcium-free solution (Ｐ＜0.01), but the IAS was not affected. Ryanodine, which can exhaust the intracellular calcium, remarkably depressed the contractions of IAS (Ｐ＜0.01),but had no effect on the colonic smooth muscles. It can be concluded that the contractions of colonic smooth muscles are mainly related to the influx of extracellular calcium; the release of intracellular calcium plays an important role in the contractions of IAS.