Objective To evaluate the clinical and histopathological features of diffuse choroidal melanoma. Methods The clinical and histopathological data of 11 patients with diffuse choroidal melanoma were reviewed retrospectively. Those patients were referred to Tianjin Eye Hospital because of visual loss or ophthalmalgia (10 cases), or Coats disease with secondary glaucoma and atrophy bulbi (1 case). The clinical disgnosis included choroidal tumor or melanoma (8 cases), absolutestage glaucoma (2 cases) and atrop hy bulbi with Coats disease (1 case). Nine patients received enucleation, and 2 patients received enucleation combined with orbital exenteration. The cellular proliferation was assessed by Ki-67staining. Results All 11 tumors had grown flatly with a wide base ranged from 12 to 20 mm, and tumor thickness ranged from 2 to 4 mm. There were 9 cases of mixed cell type, 1 case of epithelioid cell type and 1 case of necrotic cell type. The tumors invaded into the sclera in 7 cases and orbital cavity in 3 cases. Secondary glaucoma was found in 7 cases. On average, 9% (7%13%) of tumor cells were Ki67 positive and most of them located at the tumor base. There were more Ki67 positive epithelioid tumor cells than Ki67 positive spindle-shaped cells. Conclusions Diffuse choroidal melanoma had a special growth pattern and is difficult to be recognized, sometimes could be misdiagnosed as glaucoma or other choroidal tumors. With its wide base, this tumor could easily invade the orbit and metastate, and its prognosis is very poor.
目的 研究细胞视黄酸结合蛋白（CRABP）Ⅱ、表皮型脂肪酸结合蛋白（E-FABP）和Ki-67在乳腺浸润性导管癌中的表达情况及三者的相关性。 方法 采用免疫组织化学检测2001年1月－2007年12月手术切除的152例乳腺浸润性导管癌中CRABPⅡ、E-FABP和Ki-67的表达。 结果 在浸润性导管癌中，CRABPⅡ在Ki-67阴性组的阳性率高于Ki-67阳性组（P＜0.05），相反地，E-FABP在Ki-67阳性组的阳性率高于Ki-67阴性组（P＜0.05）。CRABPⅡ和Ki-67表达呈负相关（rS=?0.432，P＜0.05）；E-FABP和Ki-67表达呈正相关（rS=0.842， P＜0.05）。E-FABP和Ki-67的表达具有协同性，E-FABP和Ki-67共同表达与肿瘤的转移有关（P＜0.05）。单因素生存分析显示，E-FABP的阳性表达患者、Ki-67的阳性表达患者以及E-FABP和Ki-67的共同阳性表达患者的预后差（P＜0.05）。多因素生存分析提示E-FABP的表达（RR=4.223，P=0.012）和TNM分期（RR=8.412，P=0.000）是影响浸润性导管癌患者预后的独立危险因素。 结论 在乳腺浸润性导管癌中，CRABPⅡ和E-FABP与肿瘤细胞的增殖有关，CRABPⅡ抑制细胞增殖，E-FABP促进细胞增殖。E-FABP和Ki-67在浸润性导管癌的发生、发展中起协同作用，两者的阳性表达可能对评估肿瘤的转移和患者的预后有一定价值。
ObjectiveTo explore the expression of Ki-67 antigen in the breast cancer tissues and to evaluate the relationship of the expression to biology behavior as well as prognosis of breast cancer. MethodLiteratures about relation between Ki-67 and breast cancer were reviewed. ResultsThe expression of Ki-67 in the breast cancer tissue was obviously higher than that in the adjacent to cancer tissue or normal breast tissue. The Ki-67 positive expression rate was positively correlated with pathology classification and clinical stage of breast cancer, the correlation was not consistent about the expression of Ki-67 and axillary lymph node metastasis of breast cancer. ConclusionsKi-67 is a cell proliferation nuclear antigen related to cell cycle, and its expression changes along with the change of cell cycle, it has been employed as a reliable marker of cell proliferation. The expression of Ki-67 has an important significance in early diagnosis and guiding of neoadjuvant chemotherapy as well as prognosis of breast cancer.