ObjectiveTo investigate the hand hygiene status of nursing staff in coronavirus disease 2019 (COVID-19) isolation ward, find out the difficulties and problems in hand hygiene implementation, and then put forward scientific and feasible suggestions to improve the compliance of hand hygiene.MethodsSelf-designed Questionnaire on Hand Hygiene Status of Nursing Staff in COVID-19 Isolation Ward was distributed through the Wenjuanxing, a platform to collect data. The questionnaire, which included general information, knowledge related to hand hygiene, and the status of hand hygiene in isolation ward, was distributed to the nurses working in isolation wards in Wuhan, Hubei Province from March 15th, 2020 to March 22nd, 2020.ResultsValid questionnaires were collected from 492 nurses. The difficulty in performing hand hygiene in the isolation ward was ranked ≥level 3 by 248 nurses (50.41%), the degree of which was divided into 10 levels (level 1 was no difficulty, level 10 was the most difficult). A total of 369 participants (75.00%) thought that wearing gloves for hand disinfection would damage the gloves. There were 161 participants who thought that gloves should be changed every 2 hours, accounting for the largest proportion (32.72%); while 226 participants actually changed gloves every 4 hours, accounting for the largest proportion (45.93%).ConclusionsThe difficulty of performing hand hygiene in isolation ward should be paid attention to. It is recommended to carry out further research on the replacement time of gloves.