以临床医学实验教学中心构建为依托,紧密结合实验教学新体系的构建,建立高水平的和对外开放的实验室,理论联系实际,重新构建临床医学实验教学课程体系,并根据新的临床医学实验课程体系对原有的临床医学实验教学内容和教学模式进行改进和调整。充分发挥临床医学实验教学中心的作用,促进临床医学实验室向综合性、多功能、开放性的大型实验室转变。建立临床医学实验教学实习基地,提高临床医学实验教学质量,培养学生的实践能力和创新能力,全面推进临床医学实验教学中心的建设和管理水平。
bjectiveTo study the therapeutic effect and mechanism of photodynamic therapy (PDT) to the nude mice model of pancreatic cancer by intratumoral injecting photosensitizers hematoporphyrin derivatives (HpD), hypocrellin A (HA) and 2butylamino2demethoxyhypocrellin A (2BA2DMHA).MethodsThe animal model of human pancreatic cancer was developed by injecting human pancreatic cancer cells SW1990 into the back of the nude mice. After photosensitizers HpD, HA and 2BA2DMHA was given by the intratumoral injection, the 632.8 nm HeNe laser was used to irradiate the tumor. The curative effect was recorded and factorⅧ was used in the immunohistochemical staining to study the vessel change. ResultsPDT can destroy the pancreatic neoplasm, the tumor growth rate was significantly reduced after PDT. The immunohistochemical staining showed PDT could make injury to vessel endothelial cell.ConclusionPDT can induce injuries of pancreatic cancer; vascular injury is an important way to exert function.