ObjectiveTo explore the surgical treatment for neglected posterolateral rotatory dislocation of knee joint and evaluate the effect. MethodsSixteen patients with neglected knee posterolateral rotatory dislocation treated between January 2006 and December 2010 underwent surgical treatment. Arthroscopic lysis was first performed followed by open reduction, and then ligament reconstruction or repair was carried out in order to restore its stability. Some stiff knee joint patients underwent Patients with joint stiffness were fixed with external fixator across knee for six weeks. All the patients received preoperative and postoperative imaging examination and functional scoring. ResultsTibiofemoral and patellofemoral congruence in all the 16 patients was fully restored. Joint stability was recovered with different degrees. Two patients underwent anterior cruciate ligament reconstruction and 1 underwent posterior cruciate ligament revision during the later stage, and their joint functional status at the end of follow-up was satisfying. ConclusionThe treatment for neglected knee posterolateral rotatory dislocation by combined arthroscopy with open surgery is relatively satisfying, which is a new therapeutic approach for this type of injury.
ObjectiveTo study the effect of down-regulated leptin receptor by small interfering RNA (siRNA) in inhibiting the messenger RNA (mRNA) expressions of interleukin (IL)-1β and nitric oxide (NO) of human osteoarthritis chondrocytes, in order to provide reference for basic clinical research. MethodsCartilage was harvested under sterile conditions from osteoarthritis knee joints in patients undergoing total knee arthroplasty. Human articular chondrocytes were isolated and the cells were cultured in vitro. The cells in the 3rd passage were transferred by siRNA Ob-Rb (experimental group) and blank Ob-Rb (control group), respectively. Then mRNA expressions of IL-1β and NO were tested by quantitative polymerase chain reaction at hour 24, 48 and 72 after successful transfection. ResultsThe mRNA expressions of IL-1β increased slightly and that of NO declined slightly at hour 24, 48 and 72 after transfection in the treatment group, but they all were significantly lower than those in the control group (P ＜ 0.05) , and the differences became much larger as time went on. ConclusionLeptin receptor under siRNA technology can significantly inhibit the mRNA expressions of IL-1β and NO in human osteoarthritis chondrocytes.