Objective To study the effects of survivin antisense RNA on SGC7901 cell’s apoptosis and chemosensitivity to taxotere, and to investigate its effect on the expression of multi-drug resistance gene-1 (MDR-1). Methods Survivin antisense eukaryotic vector anti-pcDNA3-svv was transfected into SGC7901 cell lines by lipofectamine and positive clones were screened out then. Survivin protein and MDR-1 mRNA were measured by western blot and RT-PCR, respectively. Apoptosis that was induced by anti-pcDNA3-svv was observed by electronic microscope, and the sensitivity of SGC7901 cell to taxotere was examined by MTT. Results The expressions of survivin protein and MDR-1 mRNA in transfected SGC7901 cells both decreased more significantly than that of non-transfected cells (P＜0.05， P＜0.01), and the indices of MDR of transfection group and non-transfection group were 0.196±0.013 and 3.126±0.019, respectively, at the late phase of apoptosis, which had a significant difference between each other (P＜0.01), IC50 of the transfected cells to taxotere was （16.7±1.98） ng/ml and that of the non-transfected cells was (55.7±1.89) ng/ml, which also had a significant difference (P＜0.01). Conclusion Surivivin antisense RNA could induce the apoptosis of SGC7901 cancer cell line and could increase the cells’ sensitivity to taxotere, which may help to reverse drug resistance.
ObjectiveTo improve recognization and effect of surgical treatment for solid pseudopapillary tumor of pancreas (SPTP) through multidisciplinary team (MDT) discussion.MethodsThe clinical data of 1 case of SPTP treated in The Second Affiliated Hospital of Chongqing Medical University were discussed by the MDT. The best operation scheme was selected and the patient was followed-up.ResultsThe preoperative CT scan showed that the occupation on the body and tail of pancreas and suggested it was the tumorous lesions. After the MDT discussion, the preoperative diagnosis was still not completely clear. However, the decision was made to resect occupation. After the operation, the pathological examination showed it was the SPTP. There was no recurrence or metastasis with the follow-up of 6 months.ConclusionsPreoperative diagnosis of SPTP is difficult, especially for atypical SPTP. Through MDT discussion, it can help to reduce misdiagnosis rate and formulate optimal surgical treatment strategy.
The clinical practice guideline on traditional Chinese medicine alone or combined with antibiotics for patients with acute upper respiratory infection in children was issued by China Association of Chinese Medicine in June 2017. Base on the evidence, consensus and experience, five manage groups, steering committee, consensus expert group, systematic review group, drafting group and secretary group, have promoted the project. The main work process refers to up-to-date clinical practice guideline definitions of institute of medicine and quick recommendations guide of guidelines international network and WHO. In order to provide readers with a better understanding of the guideline, the drafting process of guideline principle, main work process, and basis policy were described in detail in this paper. The guidelines’ scientific characteristics, accuracy and practicability were better exhibited in attempt to enhance its promotion and application.