Objective To explore the effects of exogenous ubiquitin on lung injury and serum nitric oxide ( NO) level of mice in the early stage of sepsis induced by cecal ligation and perforation ( CLP) . Methods Seventy-seven mice were devided randomly into three groups, ie. CLP Group ( n =28) , SHAM Group ( n =28) and UB Group( n = 21) . Six hours after operation, seven mice of both Group CLP and SHAM were sacrificed for lung and blood sampling. Meanwhile all mice of Group UB were injected intravenously with exogenous ubiquitin of 10 mg/kg body weight. Seven mice of each group were sacrificed at seven, eight and nine hours after operation. Lung water content and serum level of nitrate /nitrite ( NO2 /NO3 , the stable end-products of NO) , were determined. Pathological changes of lung were compared among the groups. Results Lung water content of Group UB was lower than that of Group CLP at each observational time point. Futhermore, there was a significant differentce in lung water content between Group UB and CLP at 9 hours. Pathological examination revealed that inflammatory hyperemia and infiltration of pulmonary parenchyma reduced in Group UB, compared with Group CLP. Serum NO levels of Group CLP and UB were similar at each time point. Conclusions In the early stage of murine sepsis induced by CLP, a single introvenous bolus of exogenous ubiquitin significantly decreases lung capillary vascular permeability, and probably makes a temporal reduction of acute lung injury while it has no obvious effects on serumNO level.
ObjectiveTo investigate the knowledge and attitude of medical professionals in various regions of China on obstructive sleep apnea (OSA) and to find out the influence of sleep center setting on the above results.MethodsA self-designed questionnaire based on OSAKA questionnaire was designed. A total of 630 medical staff were investigated in 7 hospitals at different levels in various regions in China. The subjects were divided into two groups according to whether they had sleep center (including sleep monitoring room) or not. Survey data were analyzed.ResultsA total of 630 questionnaires were sent out, and 590 valid questionnaires were received, and the effective response rate was 93.65%. About half of those surveyed had sleep centers in the hospitals where they worked. There was no significant difference in three attitude problems and the choice of continuous positive airway pressure and surgical treatment between the two groups (all P>0.05). Subjects whose hospital had no sleep center were more prone to select weight loss (estimated parameters=0.513, P=0.046), no smoking and wine (estimated parameter=0.472, P=0.040), avoidance of overwork (estimated parameter=0.933, P=0.000), and drug (estimated parameter=0.802, P=0.000). The average correct rate of OSA knowledge was 45.59%±20.68%. Among them, the correct rate of response to treatment measures was the highest, and the correct rate of other knowledge points was poor. The average correct rate of total accuracy, symptoms and target organ damage in subjects whose hospital had sleep center was higher than that in subjects whose hospital had no sleep center, and there were significant differences (P=0.001, P=0.012, P=0.000). There was a positive correlation between the knowledge of OSA and their attitude towards OSA, treatment and further understanding of the knowledge (r=0.247, P=0.000).ConclusionIt is necessary to strengthen propaganda and education of OSA, and the establishment of sleep center is helpful for medical personnel to know more about OSA and to develop sleep medicine.
Objective To explore the safety and efficacy for patients with central airway-pleural fistula (APF) treated by atrial septal defect (ASD) occluder. Methods This was a retrospective study. Between January 2017 and October 2021, a total of 16 patients with postoperative APF were treated with ASD occluder through bronchoscope under local anesthesia combined with sedation. The efficacy and complication were recorded during and after the procedure. Results Sixteen patients were recruited in this study and the average age was 60.7 years (range 31 - 74 years). The main etiology for APF was lobectomy/segmentectomy (n=12), pneumonectomy (n=2), radical esophagectomy (n=1) or decortication for chronic empyema (n=1). Totally, 4 fistulas were located in right main bronchus, 3 in left main bronchus, 3 in right upper bronchus, 1 in right middle bronchus, 2 in right lower bronchus and 3 in left upper bronchus. The median diameter of APF was 7.8 mm (ranged from 4 to 18 mm) and the median diameter of ASD occluder inserted was 10.0 mm (ranged from 6 to 20 mm). Successful occlusion of APF was observed in 15 patients (15/16) and 1 patient died of multiple organ failure caused by bacteremia 14 days after the procedure. Fourteen patients were recruited for long-term follow-up, on a median follow-up period of 16.2 months (ranged from 3 to 46 months). There were 12 patients of complete remission and 2 patients of partial remission and only one patient took a second operation due to the enlargement of fistula and translocation of occluder. At follow-up, 4 patients died and the reasons were directly related to the primary etiology, and no patient died due to APF recurrence. Conclusion Endobronchial closure of central APF using ASD occluder is a minimally invasive but effective modality of treatment with satisfactory long-term outcome.