PNAS-4 is a novel pro-apoptosis gene identified latetly. In recent years, there has been a large number of research reports on the basic studies about PNAS-4 in cancer gene therapy and gene therapy of PNAS-4 alone or combined with chemotherapy or radiotherapy manifested a good application prospect, but its molecular mechanisms to promote apoptosis is not clear yet. In this paper, recent research about PNAS-4 in cancer gene therapy is briefly reviewed, and recent hypotheses on its molecular mechanisms to promote apoptosis are especially elucidated. Based on its newly identified characteristics of structural domain, we made a point that PNAS-4 might regulate functions of some target protein related to apoptosis by deSumoylation as a new deSumoylating isopeptidase, and consequently promote apoptosis.
目的:探讨人凋亡相关新基因PNAS-4(hPNAS-4)基因通过脂质体转染至Lewis肺癌LL2细胞后对放射治疗的增敏作用。〖HTH〗方法〖HTSS〗:用脂质体介导的转染技术,将hPNAS-4基因导入Lewis肺癌LL2细胞中,Western blot鉴定其过表达后,观察X射线照射对其集落形成的影响;流式细胞仪检测hPNAS-4基因或／和放疗(0,1,2,4,6 Gy)对LL2细胞生长抑制及凋亡的影响。〖HTH〗结果〖HTSS〗:通过Western blot证实了hPNAS-4基因在LL2细胞中的过表达。Lip-hPNAS-4加放射治疗处理组细胞的生存能力明显降低,肿瘤细胞的克隆形成明显减少,流式细胞术检测体外培养的肿瘤细胞凋亡明显增加。〖HTH〗结论〖HTSS〗:hPNAS-4基因联合放射治疗能产生协同抗肿瘤效应。