Objective To evaluate the use value of the perineal H-shaped incision in laparoscopic peritoneum vaginoplasty. Methods Between January 2005 and July 2011, 29 cases of congenital absence of vagina underwent laparoscopic peritoneum vaginoplasty. There were 2 married cases and 27 unmarried cases, with a mean age of 22.7 years (range, 20-30 years). The patients had no vagina mouth or had a shallow depression. B ultrasound showed normal bilateral ovarian and no uterine or primordial uterus in the pelvic cavity. H-shaped incision was made between urethra and rectum for peritoneum vaginoplasty. Results All operations were successfully completed. The mean operation time was 130.5 minutes, and the mean blood loss was 73.5 mL. Primary healing of incision was achieved in all patients and no complication occurred. All the patients were followed up 1-2 years (mean, 1.5 years). At 3 months after operation, the mean length of neovagina was 9.5 cm (range, 8.2-10.5 cm); the neovagina could hold 2 fingers and the mucosa appeared so soft and smooth with normal lubrication; vaginal exfoliate cell maturation index was 50.5% ± 9.3% ; and the mean urethra-vaginal orifices spacing was 1.0 cm (range, 0.8-1.2 cm). At 1 year after operation, the mean length of the neovagina was 9.3 cm (range, 7.2-10.0 cm); the neovagina could hold 2 fingers; the vaginal exfoliate cell maturation index was 58.6% ± 8.1%; the mean urethra-vaginal orifices spacing was 1.1 cm (range, 0.9-1.3 cm). The rate of sexual satisfaction was 95%. Conclusion Use of the H-shaped incision in laparoscopic peritoneum vaginoplasty is a preferred way to reconstruct vagina for simple operation and good effectiveness.