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find Keyword "Rb基因" 6 results
  • Study on the Expression of Rb Gene in Primary Hepatocellular Carcinoma by Tissue Chip

    Objective To determine whether Rb gene is involved in the genesis of primary hepatocellular carcinoma(HCC). MethodsForty paraffin specimens of primary HCCs with corresponding adjacent liver tissues and normal liver tissues were investigated for Rb protein expression by tissue chip and SP immunohistochemical technique. ResultsLoss of Rb protein expression occurred in 17 of 40 tumor samples, whereas in 4 of 40 adjacent liver tissue samples, only 1 of 40 normal liver tissue specimens showed negative Rb staining.Rb protein deletion in HCC was higher than that in adjacent tissues and normal liver tissues (P<0.05).Rb protein deletion rate doesn’t correlated remarkably with tumor size or phathology grade of HCC (Pgt;0.05). ConclusionRb protein deletion may play an important role in the tumorigenesis of HCC.Tissue chip is an effective highthroughput technique platform for the study of tumor molecular pathology.

    Release date:2016-08-28 05:11 Export PDF Favorites Scan
  • STUDY ON THE EXPRESSION OF p16 AND Rb IN THYROID CARCINOMA

    【Abstract】Objective To demonstrate the role of p16 protein and Rb protein in the formation and progression and their relationship in thyroid carcinoma. Methods p16 and Rb from 46 patients with thyroid cancer were detected by SP immunohistochemistry. Results In thyroid carcinoma,the p16 positive rate was decreased with the decline of differentiation. There was an inverse correlation between the rates of p16 positive expression and involvement of lymph node,clinical stage and prognosis index.The Rb protein was not present in any cases as a nucleus staining. Conclusion The p16 gene is likely to play an important role in the genesis and malignant progression of thyroid carcinoma,while the Rb gene is not .

    Release date:2016-08-28 05:30 Export PDF Favorites Scan
  • 9-顺维A酸诱导肺癌细胞L78凋亡及其与Rb和Cyclin D1基因表达的关系

    目的 研究9-顺维A酸(9-cis-retinoic acid, 9-cis RA)诱导肺鳞癌细胞株L78凋亡作用及其与Rb、Cyclin D1基因表达的关系,初步探讨其作用机制. 方法 体外培养肺鳞癌细胞株L78,随机分为两组,实验组:加入浓度为5 μmol/L的9-cis RA;对照组:加入浓度为0.1%二甲亚砜.两组均培养48小时后用免疫组织化学法检测L78细胞中Rb基因表达率,用流式细胞仪技术分别检测Rb、Cyclin D1基因表达率和肿瘤细胞凋亡率, 并研究三者之间的相关关系. 结果 实验组肺鳞癌细胞株L78细胞Rb基因表达明显增强,Cyclin D1基因表达降低,细胞凋亡率增高(P<0.01).Rb基因表达率与细胞凋亡发生率呈正相关(r=0.854,P<0.05),Cyclin D1基因表达率与细胞凋亡发生率呈负相关(r=-0.812,P<0.05). 结论 9-cis RA可能通过Rb基因表达增强和Cyclin D1基因表达降低途径,使细胞明显阻滞在G0/G1期,并诱导肺鳞癌细胞株L78细胞凋亡.

    Release date:2016-08-30 06:32 Export PDF Favorites Scan
  • THREE MOSAICISMS FOR RETINOBLASTOMA GENE POINT MUTATION

    OBJECTIVE:The hereditary form of retinoblastoma(RB)is a monogenic disorder which is due to germinal mutation of RB susceptibility gene located on 13q14.The majority of hereditary RB cases transmit as a Mendelian autosomal dominant inheritance that 50% of the offspring of a carrier will inherit the disorder susceptibility gene and all carriers will develop the disorder.The authors report 3 hereditary RB families with incompleted penetrance and irregular transmission of RB phenotype. METHOD:RFLPsamp;VNTRs for analysis of haplotype and SSCPamp;direct DNA sequencing for determination of RB point mutation. RESULTS:The mosaicism of Rb gene point mutation resulted in the incompleted penetrance and irregular transmission of RB phentype. CONCLUSION:DAN-based diagnosis can be used to differentiate the hereditary and nonhereditary forms of retinblastoma but only is the direct detection of disease-causing mutation reliable for determnation of carrier and estimation of th e risk for retinoblastoma. (Chin J Ocul Fundus Dis,1996,12: 37- 40)

    Release date:2016-09-02 06:21 Export PDF Favorites Scan
  • PREPARATION OF MONOCLONAL ANTIBODY AGAINST PRODUCT OF Rb GENE

    Synthesized Rb peptide was used as an antigen to immunize the BALB/C mice. After fusing and screening with ELISA and Western Blot, we got 3 hybridomas which secreted specific monoclonal antibodies against product of Rb gene. All of three kinds of antibodies were fourld to be IgG 1 through the appraisement. In addition,since we mixed three kinds of peptides as the antigens to immunize the mice,we have got three different hinds of monoclonal antibodies including one against Rb product and another two in the Same procedure. (Chin J Ocul Fundus Dis,1993,9:2-4)

    Release date:2016-09-02 06:35 Export PDF Favorites Scan
  • RETIONBLASTOMA GENE REGULATES THE CELL CYCLE AND SUPRESSES THE GROWTH AND TUMORIGENISITY OF HUMAN BREAST CANCER CELLS

    The human wild-type Rb cDNA has been inserted into a retrovirus vector DOL and introduced into the human breast cancer cell MDAMB468,which has a large deletion of exons 3-27 of Rb genes,by electroporation transfection techniques.The exogenous Rb gene expresses the 110kd Rb protein.The morphology of the transfected cells is similar to that of the parent MDAMB468 cells.With the expression of Rb protein,the growth rate of MDAMB468 cells decreases by about 50%,and the colony formation ability in soft agaris repressed completely.After injection of 3times;106Rb+ cells and Rb-MDAMB468 cells into nude mice,the tumors formed from 106Rb+ cells are smaller than those from Rb-cells.The cell population of G1 and S phase of Rb+ MDAMB468 cells increases and the proliferation quotient decreases by about 50%.This result supports the former report the Rb protein. (Chin J Ocul Fundus Dis,1993,9:135-140)

    Release date:2016-09-02 06:35 Export PDF Favorites Scan
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