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find Keyword "Receptors, CCR3" 1 results
  • Light-induced changes of ligands of chemokine (C-C motif) receptor 3 on human RPE cells

    Objective To investigate the expression of eotaxin-1, eotaxin-2 and eotaxin-3 in ARPE-19 human RPE cells after exposure to light. Methods Cultured human RPE cells (5th~10th generations) were divided into lightinduced group and control group. Cells light-induced group were exposed to the blue light at the intensity of (600plusmn;100) Lux for 12 h to establish the light damaged model. Eotaxin-1, eotaxin-2 and eotaxin-3 mRNA and protein were determined by real time polymerase chain reaction and Western blot at 0, 3, 6, 12, 24 hours after light-induced. Results In light-induced groups, mRNA levels of eotaxin-1 and eotaxin-2 were increased at 0 h (t1=6.05.t2=12.561) and 3 h (t1=2.95.t2=3.67) significantly(P<0.05), but the mRNA level of eotaxin-3 had not changed (t3=1.57 and 1.00 respectively,P>0.05) at that time. At 6 h (t1=4.73,t2=18.64,t3=28.48), 12 h (t1=3.11,t2=20.62,t3=18.50), 24 h (t1=8.25,t2=38.27,t3=18.60), mRNA levels of eotaxin-1, 2, 3 were increased significantly (P<0.05). Except for the eotaxin-3 protein had not changed at 3 h (t3=1.28,P>0.05), protein expression of eotaxin-1, 2, 3 were increased significantly (P<0.05) at 0 h (t1=4.85,t2=5.45,t3=6..21), 3 h (t1=5.64,t2=4.55), 6 h (t1=31.60,t2=6.63,t3=7.15), 12 h (t1=14.09,t2=18.22,t3=15.76), 24 h (t1=6.96,t2=10.47,t3=12.85). Conclusion Eotaxin-1, eotaxin-2 and eotaxin-3 expression were increased after Light-damage, corresponding to the time after light exposure. Eotaxin-3 was the most prominent isoform.

    Release date:2016-09-02 05:22 Export PDF Favorites Scan
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