目的 研究尿标本中防腐剂盐酸对24 h尿游离皮质醇测定的影响。 方法 收集2008年7月-2009年1月正常人、库欣病患者及其他疾病患者的24 h尿液，混匀后，一部分浓盐酸防腐，一部分未加盐酸直接保存。电化学发光免疫分析法同步检测尿游离皮质醇浓度。 结果 经配对 t 检验加浓盐酸后的24 h尿游离皮质醇测值均高于未加酸者，比较有统计学意义（Plt;0.05）。加盐酸和未加盐酸所测尿游离皮质醇二者之间具有较好的相关性，相关系数 r =0.97，P lt;0.05。 结论 浓盐酸防腐的标本24 h尿游离皮质醇测值较未加酸保存的标本高。因此，为了得到相对准确的值，更好地反映肾上腺实际分泌情况，测定24 h尿游离皮质醇的标本不应使用盐酸防腐。
ObjectiveTo detect the 2019 novel coronavirus (2019-nCoV) in various biological specimens of novel coronavirus pneumonia (NCP), and preliminarily observe the status of 2019-nCoV in different systems of the body and its clinical significance.MethodsThe study design was a small-scale cross-sectional observational study. All the confirmed NCP cases being treated in the Second People’s Hospital of Yibin · West China Yibin Hospital, Sichuan University on February 2nd, 2020 were enrolled in this study. Two sets of primers were designed for 2019-nCoV-1ab and N regions using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay. The 2019-nCoV in upper respiratory specimens, blood, feces and urine specimens of the NCP cases were detected on the single day.ResultsA total of 7 imported NCP cases (mild type) were included. The 7 patients were confirmed by the positive results of 2019-nCoV nucleic acid tests of upper or lower respiratory specimens between the 3rd day and the 7th day after fever onset, while 2 patients were found positive on the 3rd day after onset. The 2019-nCoV nucleic acid tests of the 7 patients were detected again on a single day between the 7th day and the 15th day after onset, and the results showed: the upper respiratory specimens of 5 patients were found negative (1 case was on the 7th day after onset); 2019-nCoV was not detected in the blood, feces or urine specimens of the 7 patients.ConclusionsFor mild type NCP patients, real-time RT-PCR test could detect 2019-nCoV between the 3rd day and the 7th day after onset, while 2019-nCoV might become negative since the 7th day after onset. 2019-nCoV was not detected in the blood, feces or urine of mild type NCP patients on the single day between the 7th day and the 15th day after onset. This study was only a preliminary observational study, which needed high-qualified studies to obtain more definitive conclusions.