Objective To explore the incidence of lipohypertrophy in patients with diabetes by meta-analysis, and provide a reference for preventing and reducing its occurrence. Methods A computer search was performed on PubMed, Embase, Web of Science, China National Knowledge Infrastructure, Wangfang Data and Chongqing VIP databases, supplemented by literature retrospective method and manual search. Relevant documents related to lipohypertrophy in diabetic patients were searched until September 2021. After conducting independent literature screening, data extraction and quality evaluation, the researchers used RevMan 5.4.1 and R 3.3.1 softwares for meta-analysis. Results Ten articles were included, including 0 in Chinese and 10 in English, and 20277 patients in total. The incidence of lipohypertrophy in patients with diabetes was 53% [95% confidence interval (44%, 62%)]. The heterogeneity among studies was statistically significant (I2=99%, P<0.00001). Meta-regression analysis of single covariates and multiple covariates found that publication year, research scope, research area, sample source, research center, and sample size could explaine 51.6% of the heterogeneity. Conclusion The incidence of lipohypertrophy in patients with diabetes is high, and effective intervention measures are urgently needed to prevent, diagnose and treat the problem of lipohypertrophy in patients with diabetes.