Objective To review the relationship between the expression levels of bcl-2, bax and bad gene and other biological factors of breast cancer in the growth and development of breast cancer. Methods Related literatures were summarized and reviewed. Results The expression level change of antiapoptosis gene bcl-2 was still under research and the expression levels of apoptosis gene bax and bad were down-regulated progressively in the evolution from benign breast tissue to breast cancer tissue. The expression level of bcl-2 had positive correlation with some positive factors in breast cancer such as estrogen receptor （ER） and progesterone receptor (PR), while it had negative correlation with some negative factors such as p53, EGFR, c-erbB-2 and lymph node metastasis. The levels of ER, PR and the expression level of p53 of breast cancer had no relationship with the expression level of bax. Up to now there was no report about the relationship between the expression level of bad and other biological factors of breast cancer. Conclusion The role of altered expression level of bcl-2, in the treatment and prognosis of breast cancer is still controversial, and the relationship between the expression of bad and the prognosis of breast cancer is still unknown, but expression level of bax is correlated positively with the prognosis of breast cancer. Research on these genes can provide us some new index to evaluate the prognosis of breast cancer and new ideas on treatment of breast cancer including gene therapy.
【Abstract】Objective To investigate the effect of donor blood transfusion on inducing pancreatic allograft tolerance in outbred rat model. Methods Wistar male rats were used as blood and pancreas donor, and diabetic recipients. One ml of donor blood injected into abdomen of diabetic recipients on the day of transplantation and azathioprine given 2 days pretransplant and continued for three days. Results Pancreas allograft survival was significantly prolonged (28 to 112 days, media survival time 64.2 days). One ml of donor blood alone injected into the abdomen and azathioprine given alone 2 days pretransplant did not improve allograft survival (media survival time 9.8 vs 10.2 days). Conclusion Donor blood injected on the day of transplantation and a 3 days course of azathioprine started 2 days pretransplant have b synergism in inducing long term graft survival in this rat model.
【摘要】 目的 观察手术前后乳腺癌患者体内辅助性T淋巴细胞（helper T lymphocyte，Th） 1、2型细胞因子的表达及意义。 方法 2009年11月－2010年12月，采用酶联免疫吸附试验检测40例乳腺癌患者（乳腺癌组）手术前、后和15例健康妇女（正常对照组）外周血单个核细胞培养上清液中Thl型细胞因子[白细胞介素（interleukin，IL）-2、干扰素（interferon，IFN）-γ]和Th2型细胞因子（IL-4、IL-6）表达水平。 结果 乳腺癌组术前外周血IL-2、IFN-γ、IL-4和IL-6的检测结果在分别为（326.5±67.4）、（288.5±35.6）、（41.2±10.8）、（526.1±136.4） ng/L，手术后分别为（958.1±284.7）、（559.6±106.9）、（26.1± 8.7）、（395.6±125.9） ng/L；在正常对照组分别为（1 498.0±325.9）、（784.6±126.3）、（16.6± 3.7）、（297.4± 83.7） ng/L。与正常对照组相比，术前乳腺癌患者外周血Th1型细胞因子表达降低，Th2型细胞因子表达升高，差异有统计学意义（Plt;0.05）；与术前相比，乳腺癌患者术后Thl型细胞因子表达升高，Th2型细胞因子表达降低，差异有统计学意义（Plt;0.05）。 结论 ①乳腺癌患者Th细胞呈现Th1抑制和Th2极化状态，细胞免疫受抑制。②手术切除肿瘤可在早期一定程度上逆转Th1/Th2的失衡状态，改善机体免疫状态，对预后产生积极作用。【Abstract】 Objective To investigate the expression and significance of helper T lymphocyte(Th) 1 and 2 of breast cancer patients in vivo before and after operation. Methods From November 2009 to December 2010, Thl-type cytokines [interleukin-2 (IL-2), interferon-γ (IFN-γ)] and Th2-type cytokines (IL-4, IL-6) expression in supernatants of peripheral blood mononuclear cells (PBMC) were detected by enzyme-linked immunosorbent assay (ELISA) in 40 breast cancer patients before and after surgery (Breast cancer group) and 15 healthy women (Control group). Results The test results of IL-2, IFN-γ, IL-4 and IL-6 in peripheral blood for patients of breast cancer group were (326.5±67.4), (288.5±35.6), (41.2±10.8), and (526.1±136.4) ng/L before surgery, and (958.1±284.7), (559.6±106.9), (26.1±8.7), and (395.6±125.9) ng/L after surgery. Those results for patients in the normal control group were (1498.0±325.9), (784.6±126.3), (16.6±3.7), and (297.4±83.7) ng/L respectively. Compared with the control group, the expression of peripheral blood Th1-type cytokines in pre-operative breast cancer patients was significantly lower, while expression of Th2-type cytokines was significantly higher (Plt;0.05); Compared with the pre-operative data, the expression of peripheral blood Th1-type cytokines in post-operative breast cancer patients was significantly higher, and the expression of Th2-type cytokines was significantly lower (Plt;0.05). Conclusions ①For Th cells in breast cancer patients, there is a tendency of Th1 suppression and Th2 polarization, and cellular immunity is suppressed. ②To some extent, surgical removal of tumors can reverse the Th1/Th2 imbalance early, improve the immune status, and has a positive effect on the prognosis.
【Abstract】ObjectiveTo study the expressions of P-gp, nm23 and p53 in primary breast cancer tissues and to evaluate the prediction significance in recurrence of the primary breast cancer. MethodsExpressions of P-gp, nm23 and p53 in 57 benign and malignant breast paraffin-embedded specimens, the difference of the rate and intensity of positive reaction of P-gp,nm23 and p53 were checked by immunohistochemical staining.ResultsThe positive rate of nm23 significantly increased in benign breast tissues compared with the tissues of recurrence of breast cancer. The positive intensity of nm23 was significantly decreased in the tissues of recurrence of breast cancer compared with the tissues of primary breast cancer. The positive rate of p53 was significantly increased in malignant breast tissues compared with benign breast tissues. The positive intensity of p53 was significantly increased in the tissues of recurrence of breast cancer compared with the tissues of primary breast cancer. There was no difference in the positive rate of P-gp in the tissue of recurrence and primary of breast cancer compared with benign breast tumor. But the positive intensity of P-gp was significantly decreased in the tissues of recurrence and primary of breast cancer compared with benign breast tumor. ConclusionThe expression of p53 may indicate the proliferating activity of carcinoma cell. The positive intensity of expression of p53 and nm23 is valuable in prediction of the recurrence of primary breast cancer.
Ginger moxibustion has the effect of regulating zang-fu organs and activating qi and blood circulation. When used, ginger paste is required to be close to human skin. Currently, the ginger box used clinically in the hospital can't meet the requirement of large area fitting human skin, and the efficacy of ginger moxibustion is significantly reduced. In this study, a flexible ginger paste box was proposed, which was composed of flexible components polydimethylsiloxane (PDMS), spring and wire netting. The large flexibility of the structure made it fit well with human skin. Finite element method was used to study the fitting degree between ginger paste box and waist soft tissue. Finite element models of flexible ginger paste box and waist soft tissue were established based on Hypermesh and Abaqus software. The equivalent contact area between the flexible ginger paste box and waist was obtained by numerical simulation under different PDMS unilateral thickness, spring wire diameter, wire netting diameter and ginger paste layer thickness. The four parameters were taken as the influencing factors, and the equivalent contact area was taken as the optimization objective. The typical value analysis and variance analysis of S/N were performed by Taguchi method, and the results showed that among the four influencing factors, the wire netting diameter had the largest influence on equivalent contact area and its contribution rate reached 41.98%. The contribution rates of PDMS unilateral thickness, spring wire diameter and ginger paste layer thickness reached 36.48%, 13.97% and 6.50%, respectively. The optimized PDMS unilateral thickness, spring wire diameter, wire netting diameter and ginger paste layer thickness were 1.5, 0.4, 0.15, 35 mm, respectively, and the equivalent contact area was 95.60 cm2. The optimized flexible ginger paste box with great fitting performance can improve the effect of ginger moxibustion.