ObjectiveTo compare the properties of hernia repair mesh of different materials used in clinical practice, so as to provide reference for clinical selection of numerous meshes at present. MethodThe literatures of different materials of hernia mesh in the treatment of abdominal hernia and inguinal hernia at home and abroad were reviewed.ResultsThe synthetic mesh, especially the polypropylene mesh with wide-pore mesh in the midweight mesh, might be the most ideal synthetic mesh. The new coating material improved the defect of polypropylene material to a great extent. The status of biological mesh in complex hernia repair had been waxed, and it had a tendency to be replaced by absorbable synthetic mesh.ConclusionsCurrently, there is no single mesh suitable for all types of hernia repair. Polypropylene mesh is still an effective and low cost mesh material, and there may be more room for the development of absorbable synthetic materials and antimicrobial coatings.
Objective To establish the heterologous recombinant embryonic stem cell （ES cell） with ghrelin receptor (GHS-R) gene deletion in order to study the function of the GHS-R gene. Methods PGK-neo cassette was replaced by TK-neo in X-pPNT agent. The target region was located in exon 1 and exon 2 of GHS-R. Two homologous arms were amplified from mouse ES cells genomic DNA and constructed into X-pPNT with SalⅠ/NotⅠand EcoRⅠ/BamHⅠ, respectively. ES cells were electrotransfected with the linearized targeting vector and screened with G418 and Gancyclovir. Finally, the positive ES cell clones were identified by PCR and sequencing. Results The X-pPNT-TK-neo vector was obtained. And two homologous arms were inserted correctly. Finally, 328 positive clones were obtained by G418 and Gancyclovir screening, and 3 clones were confirmed as GHS-R gene homologous recombination. Conclusion This study provides the necessary basis for the establishment of the GHS-R knock out mouse model and the further study on GHS-R gene function in vivo.
ObjectiveTo understand the research progress of Aurora kinase A (AURKA) in colorectal cancer, so as to provide new ideas for treatment of colorectal cancer. MethodThe relevant to literature on AURKA and its relation with colorectal cancer was searched and reviewed. ResultsThe AURKA was a member of the polygenic family of mitosis/threonine protein kinases. The studies in recent years had found that the AURKA not only played an important role in the regulation in the cell cycle processes, but also played a different role outside the cell cycle. The AURKA abnormally expressed in a variety of malignancies, including colorectal cancer, and was associated with a poor prognosis in patients, and many inhibitors against AURKA were developed and evaluated at different stages of clinical study. ConclusionAs a key gene in the occurrence and development of colorectal cancer, AURKA should be further studied to clarify its specific mechanism of action, and targeted drugs may be developed.
Objective To find and evaluate the existence of distant peritoneal micrometastasis of gastric cancer in rectovesical pouch or Douglas pouch by using immunohistochemist ry method. Methods Forty cases of gastric cancer were collected f rom June 2004 to March 2006 in Nanjing Gulou hospital . None of them showed obvious distant peritoneal metastasis in preoperative physical and imaging examinations and laparotomy inspection or palpation. Tissues were taken f rom rectovesical pouch or Douglas pouch during the operations , and HE and CEA/ CK220 immunohistochemistry staining were then performed on the tissues. Results Distant peritoneal micrometastasis in rectovesical pouch or Douglas pouch were found in 10 cases out of the 40 cases , all of which were found to have full-thickness invasion or invasion out side gast ric serous tunic 〔27. 8 % (10/ 36) 〕. Their occurrence rates of peritoneal micrometastasis were significantly higher than those without full-thickness invasion〔0 (0/ 4) 〕, Plt;0. 05. The number of metastatic lymph nodes was more than six in 8 cases , was only one in 2 case , the occurrence rate of peritoneal micrometastasis of the number of metastatic lymph nodes was more than seven 〔44. 4 %(8/ 18) 〕which was significantly higher than that the number was less than seven〔16. 7 % (2/ 12) 〕, Plt;0. 05. In 10 cases , 8 cases were poorly differentiated adenocarcinoma , and the other two were moderately differentiated. Conclusion When gast ric carcinoma invaded serous tunic or outside , though peritoneal metastasis may not be found by preoperational inspection or intraoperative palpation , peritoneal biopsy in rectovesical pouch or Douglas pouch may be necessary to perform as a routine procedure to detect distant peritoneal micrometastasis. It may be useful for staging , adjuvant chemotherapy and prognosis forecast.
Objective To explore the value of multi-slice spiral CT (MSCT) 3D imaging in evaluating the efficacy of neoadjuvant chemotherapy for advanced gastric cancer. MethodsSixty-one patients with gastric cancer diagnosed by gastroscopy and pathological examination at the First Hospital of Lanzhou University from January 2019 to March 2022 were divided into chemotherapy effective group (n=39) and ineffective group (n=22) according to postoperative pathological regression grade (tumor regression grade, TRG) standards. MSCT was performed before neoadjuvant chemotherapy and before undergoing surgical treatment after neoadjuvant chemotherapy. The independent predictors related to the efficacy of chemotherapy were screened by binary logistics regression analysis of CT conventional observation indexes (including maximum tumor thickness, gastric wall motility, enhancement mode, lymph node metastasis, distant metastasis, peritoneal thickening or peritoneal nodules). Tumor volume and maximum tumor thickness were measured with the imaging histology software ITK-snap, and the diagnostic efficacy of tumor volume and CT conventional observation indexes was analyzed. Results In the evaluation of chemotherapy efficacy, tumor volume reduction rate and tumor maximum thickness reduction rate can evaluate the efficacy of chemotherapy to a certain extent (P< 0.01). The statistically significant indicators (tumor maximum thickness reduction rate, gastric wall motility, lesion intensification mode and peritoneal thickening and nodules) were analyzed by univariate analysis and binary logistic regression. The results showed that gastric wall motility [OR=0.294, 95%CI (0.093, 0.928), P=0.037] and maximum tumor thickness reduction rate [OR=0.282, 95%CI (0.083, 0.957), P=0.042] were independent predictors of the efficacy of neoadjuvant chemotherapy for progressive gastric cancer. Receiver operating characteristic (ROC) curve were plotted based on the predicted probability variable obtained from both and the results showed that the area under curve (AUC=0.900) , sensitivity (83.3%), and specificity (99.8%) of the tumor volume reduction rate were all higher than those of CT clinical index prediction probability variables (AUC=0.802, sensitivity was 58.3%, specificity was 85.7%). ConclusionThe measurement of tumor volume by MSCT combined with the imaging omics software ITK-snap provides an objective basis for the prediction of the efficacy of neoadjuvant chemotherapy, and its diagnostic efficacy is better.
Objective To summarize the research progress of copper and its derivatives in gastrointestinal tumors in recent years, aiming to provide reference for clinical diagnosis and treatment decisions. Method The literatures related to copper homeostasis and copper death in recent years were read and summarized, and the research progress on the role of copper in cancer and copper applications in cancer diagnosis and treatment was reviewed. Results Copper was an essential trace element involved in a variety of basic biological processes. Elevated levels of copper in serum and tissues were associated with the development of tumors. As the mechanisms of copper action in various gastrointestinal tumors were being investigated, the use of copper and related derivatives in the treatment of cancer patients had become a new strategy. Conclusion Copper and its derivatives have a promising future in the treatment of gastrointestinal tumors, but their benefits in clinical patients still need to be demonstrated in numerous clinical trials.