Patch-clamp is used to study all sorts of ionic channels and their regulations with measuring pA current of cell ionic channel, but the fast capacitance (C-fast) compensation and slow capacitance (C-slow) compensation transient currents are caused by measuring objects and measuring instruments themselves which will change the properties of action potentials. The present paper firstly discusses the C-Fast transient currents affecting membrane capacitance and membrane potential, and then draws a conclusion that the changes of membrane potential affect the properties of action potential through analyzing the changes of membrane potential in H-H model. Based on this conclusion, we discuss the influence mechanisms mainly through the analysis of traditional C-fast compensation errors, and focus discussion on the shape of electrode capacitance affecting C-fast. This method can not only improve the compensation speed greatly, but also improve the compensation precision from the electrode shape as much as possible.
In order to realize sleep staging automatically and conveniently, we used support vector machine (SVM) to analyze the correlation between heart rate variability and sleep stage experimentally. R-R intervals (RRIs) from 33 cases of sleep clinical data of Tianjin Thoracic Hospital were extracted and analyzed by principal component analysis (PCA). The SVM method was used to establish the model and predict the five sleep stages. The prediction accuracy of three-sleep-stage was higher than 80%, in contrast to sleep scoring annotations marked by physiological experts based on electroencephalogram (EEG) golden standard. The result showed that there was a good correlation between heart rate variability and sleep staging. This method is an important supplement to the traditional sleep staging method and has a great value for clinical application.