Obiective lt;brgt;To investigate the change of the activity of proliferation in cultivated Muuml;ller cells treated by advanced glycation endoproducts (AGEs), and the effect of these changes on expression of occludin in bovine retinal vascular endothelial cells (BREC). lt;brgt;Methods lt;brgt;The cultivated Muuml;ller cells were devided into normal growth group and cultured with AGEs group. The cultured BREC were devided into 4 groups:group 1, without any medium; group 2, with normal growth Muuml;ller cell medium (MCM); group 3,MCM treated by AGEs; group 4, without cell as the control. Enzyme-linked immuno sorbent assay was used to detect the content of occludin in the medium in the 4 groups. lt;brgt;Results lt;brgt;The content of expression of occludin was the most in group 2, less in group 1, and the least in group 3. lt;brgt;Conclusion lt;brgt;AGEs may promote the abnormal proliferation of Muuml;ller cells and inhibit the expression of occludin in BREC. lt;brgt;(Chin J Ocul Fundus Dis, 2006, 22: 28-30)
Purpose To investigate the histopathologic changes of macula lutea retinae in the elderly Chinese population,and to provide information for the cause of visiual disturbance in an autopsy study. Methods Two hundred and twelve eyes from 108 consecutive cases of postmortem (mean age of 78.4 years old) sections of the area of macula lutea retinae were studied by histopathology. Results Among the 212 eyes,hard drusen were found in 36.3% of eyes and soft drusen in 19.3% of eyes.The eyes with both of the above mentioned 2 types of drusens were found to be combined with RPE atrophy and RPE detachment;and subretinal neovascularization were found in 5.7% of these eyes and they were associated with obvious RPE atrophy and photoreceptor loss;3.2% eyes had posterior scleral staphyloma showing thinning of the sclera and choroid,RPE atrophy and apparent photoreceptor loss;2.4%eyes showed CME with the history of cataract surgery. Conclusion The macular affections,age-related macular degeneration,myopic macular degeneration and post-perative cystoid macular edema are varying in number in the above mentioned order successively,in elderly Chinese at an autopsy study. (Chin J Ocul Fundus Dis,2000,16:233-235)
PURPOSE:To evaluate the ability Of retinoic acid(RA) in silicon oil(SiO)to inhibit the proliferation of injected intraocular fibroblast cells. METHODS:Thirty New Zealand white rabbits (58 eyes)were divided into three groups. In control group ,only SiO(10 eyes)or BSS(10 eyes)were injected intravitreally and 5mu;g/ml (18 eyes)or 10mu;g/ml (20 eyes)RA in SiO were injected into other lwo groups respectively. Three days after gas-compression vitrectomy, 2 times;105 fibroblasts and Sio(0.5ml)or BSS(0.5ml)were injected in all eyes sequentially.The morbidity of tractional retinal detachment (TRD) were observed by ophthalmoscope until 4 weeks. RESULTS:After 4 weeks,in control ,5mu;g/ml RA in SiO and 10mu;g/ml RA in SiO group,80. 00%,44.44%,and 30.00% eyes developed TRD respectively. Significant statistical differences were found between the control group and the two treated groups (P＜0.05). CONCLUSIONS:5mu;g/ml or 10mu;g/ml RA in SiO can inhibit the occurrence of TRD effectively. （Chin J Ocul Fundus Dis,1997,13:174-176）
OBJECTIVE:To evaluate the toxicity of retinoic acid in silicone oil to the retinal tissue. METHOD:Twelve New Zealand white rabbits(24 eyes)were divided into three grorps at random. Three days after gas-compression vitrectomy,24 eyes were unedrgone gas/silicone oil exchange. The silicone oil 0.5 ml was injected intravitreally in 4 eyes as controls ,and 5mu;g/ml retinoic acid silicone oil 0.5ml in 10 eyes and 10 mu;g/ml retinoic acid silicone oil 0.5 ml in 10 eyes respectively as 2 study groups. After intravitrea[ injections, all the eyes were examined by ophthalmoscopy on the 1st, 3rd, 7th, 14th, 21st and 28th day. The retinas of the enucleated eyes on the 28th day were then examined by light microscopy and transmission electrone microscopy. RESULT: No evidence of toxicity was found in retinas after intravitreal injections of silicone oil with 5 mu;g/ml or 10 mu;g/ml retinoic acid. CONCLUSION :There was no toxic effect on the retinas by using 5 mu;g/ml or 10 mu;g/ml retinoic acid in intravitreal silicone oil tamponade operation. （Chin J Ocul Fundus Dis,1997,13: 81-82）