ObjectiveTo investigate expression and clinical significance of long chain noncoding RNA POU6F2-AS2 in human gastric cancer tissues.MethodsSeventy-three pairs of human gastric cancer and matched paracancerous tissues from May 2017 to May 2018 in the Affiliated Hospital of North Sichuan Medical College were collected. The real-time fluorescence quantitative polymerase chain reaction was used to detect the expression level of POU6F2-AS2 in the gastric cancer tissue and its paracancerous tissue. The correlations between its expression level and clinicalpathologic features of patients were analyzed by the chi-square text. The relationship between the expression of POU6F2-AS2 and the overall survival rate of patient with gastric cancer was analyzed by the Kaplan Meier Plotter database data.ResultsThe relative expression of POU6F2-AS2 in the gastric cancer tissues was significantly higher than that in the corresponding adjacent tissues (P<0.050). The patients were divided into the high expression (43 cases) and low expression group (30 cases) according to the expression level of POU6F2-AS2 in the gastric cancer tissues and their corresponding adjacent tissues. The results of the relationship between the expression of POU6F2-AS2 and the clinicopathologic characteristics of patients with gastric cancer showed that the POU6F2-AS2 expression was significantly correlated with the depth of invasion (P=0.022) or the TNM stage (P=0.032). There were no significant differences in the gender, age, smoking history, drinking history, tumor diameter, degree of differentiation, lymph node metastasis, distant metastasis, vascular invasion, nerve invasion, liver metastasis, ascites, and fat nodule metastasis (P>0.050). The overall survival rate of high expression of POU6F2-AS2 in the patient with gastric cancer was significantly worse than that of the low expression of POU6F2-AS2 by the Kaplan Meier Plotter database.ConclusionsHigh expression of POU6F2-AS2 is related to depth of tumor invasion and TNM stage, which indicates that POU6F2-AS2 might play an important role in regulating occurrence and development of gastric cancer. It may be used as an important target for gene diagnosis and treatment of gastric cancer and as a biomarker for evaluating prognosis of patients with gastric cancer.