ObjectiveTo study the value of magnetic resonance multiple sequence examination in the diagnosis of biliary sludges. MethodsThe display rates of biliary sludges were obtained from 56 cases of biliary sludges between February 2011 and March 2013 examined with trans T1 weighted imaging (T1WI), T2 weighted imaging with fat suppression (T2WI-FS), true fast imaging with steady-state precession (True FISP), and MR cholangiopancreatography (MRCP) proved by operations or endoscopic retrograde cholangio-pancreatography. Then we carried out a statistical comparison study. ResultsThe display rate combined with trans T1WI, T2WI-FS, True FISP, and MRCP sequence was statistically higher than any other single sequence (P<0.005). Diagnostic sensitivity, specificity and accuracy of MRI for biliary sludges were 85.7%, 84.1% and 84.5%, respectively. ConclusionTo improve the display rate of biliary sludges on MRI, multiple sequences including trans T1WI, T2WI-FS, True FISP, and MRCP should be used.
ObjectiveAfter establishing the rabbit brain death model, TUNEL, western blotting, and immuno-histochemical methods were used to detect hepatocyte apoptosis to study hepatocyte apoptosis level from rabbit donors after brain death. MethodsSixty healthy male New Zealand rabbits were divided into brain death group (n=30) and sham group (n=30). The rabbits of brain death group were established by increasing intracranial pressure in a modified, slow, and intermittent way, collecting liver tissues after corresponding treatment respectively, using TUNEL to detect apoptosis rate, western blotting and immunohistochemical methods to detect the expression of Cleaved-caspase 3. ResultsThe hepatocyte apoptosis rate at each time point of brain death group were higher than those of the corresponding time point of sham group (P<0.05), and the rate of hepatocyte apoptosis increased gradually with the extension of brain death time (P<0.05). The results of Western blot assay and immunohistochemistry assay showed that the relative expression amount of Cleaved-caspase 3 protein increased gradually with the extension of brain death time (P<0.05), and relative expression amount of Cleaved-caspase 3 protein at each time point of brain death group were higher than those of the corresponding time point of sham group (P<0.05). ConclusionsThe relationship between brain death donor liver and cell apoptosis is closely related. Along with the extension of the brain death time in rabbits, the level of apoptosis of liver cells gradually increased, which affects the quality of liver donors after brain death.