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葡萄膜与结膜色素性肿瘤DNA含量的定量研究

PURPOSE: To evaluate the value of cellular DNA content and proliferative activity in an attempt to distinguish between benign and malignant or to estimate malignant potential of conjunetival and uveal pigmental tumors. METHODS :Cellular DNA content ,proliferative index(PI) and s-phase fraction(SPF)from uveal malignant melanomas (UMM),conjunctival nevi (CN) and malignant melanomas (CMM) were measured by flow cytometry(FCM). RESULTS:Seven cases of CN were diploid,aneuploid incidence of 7 cases of CMM and 39 cases of UMM was 57.14% and 74.36% respectively. SPF and PI in CMM were significantly higher than SPF (Plt;0.05) and PI(Plt;0.01)in CN;DNA index (DI) and aneuploid incidence in UMM were related to different pathological category (Plt;0.01). In 19 cases of UMM,there was a significantly positive correlation between DI and argyrophilic nucleolar organizer regions (AgNORs) asssociated proteins areas (Plt;0.01). CONCLUSIONS: It is suggested that aneuploid might be a characteristic feature of CMM and UMM ;SPF and PI might be used as adjunctive indexes in distinguishing between benign and malignant conjunctival melanomas :DI and silver-stained areas replenish each other to provide tumor biological information in different aspects. （Chin J Ocul Fundus Dis,1996,12: 94- 97）