目的 建立测定胎盘灌流液中格列苯脲浓度的高效液相色谱（HPLC）检测方法。方法 采用的色谱柱为Symmetry Shield RP C18（150 mm×4.6 mm，5 μm），柱温40℃，流动相为NaH2PO4缓冲盐（25 mmol/L，pH值5.2）︰乙腈=1︰1；内标为格列齐特，流速1.0 mL/min，检测波长228 nm，采用液-液萃取预处理方法测定胎盘灌流液中格列苯脲的浓度。 结果 格列苯脲浓度线性范围为2.0～25.0 μg/mL，线性方程为：y=0.226x+0.002，r=0.999 9 （n=6），日内相对标准偏差（RSD）＜3.1%，日间RSD＜9.5%，方法学回收率为95.32%～103.35%。 结论 HPLC检测方法灵敏、简便，可用于胎盘灌流液中格列苯脲浓度的检测。
目的 建立柱前衍生化反相高效液相色谱-荧光检测法测定血浆中同型半胱氨酸（Hcy）浓度的方法。 方法 以tris-(2-carboxylethyl)-phosphine hydrochloride （TCEP）为还原剂，以7-fluorbenzo-2-oxa-1,3-diazole-4-sulfonate （SBD-F）为衍生剂，色谱柱为Xterra RP18柱，柱温35℃，流动相为甲醇︰磷酸二氢钠缓冲液（pH值3.0）=3︰97，激发波长380 nm，发射波长510 nm，外标法定量。 结果 Hcy浓度在1.95～125 ?mol/L范围内线性关系良好（r=0.999 8）。日内和日间相对标准偏差均＜7%，方法回收率为103.2%～111.9%。 结论 此方法准确、灵敏、快速，是一种适合于实验室研究和临床检测血浆中Hcy浓度的方法。
ObjectivesTo systematically review the efficacy and safety of escitalopram in the prevention of post-stroke depression (PSD).MethodsPubMed, The Cochrane Library, CBM, WanFang Data, VIP and CNKI databases were electronically searched to collect randomized controlled trials (RCTs) on escitalopram in preventing PSD from inception to March 2019. Two reviewers independently screened literature, extracted data and assessed the risk of bias of included studies. Then, meta-analysis was performed by using RevMan 5.3 software.ResultsA total of 6 RCTs involving 891 patients were included. The results of meta-analysis showed that: compared with the control group, the escitalopram group could reduce the incidence of PSD (RR=0.55, 95%CI 0.31 to 0.98, P=0.04). In addition, there was no statistical difference between escitalopram group and control group in rate of adverse events (P≥0.05).ConclusionsCurrent evidence shows that escitalopram can reduce the incidence of PSD without increasing the incidence of adverse reactions. Due to limited quality and quantity of the included studies, more high quality studies are required to verify above conclusions.