Objective To investigate the relationship between the polymorphism of 7α-hydroxylase (CYP7A1) and cholestero1 cholecystolithiasis. Methods CYP7A-1 genotyping was performed by PCR-RFLP approach in 160 cholesterol cholecystolithiasis patients and 94 control subjects.Results The frequencies of C, A allele of CYP7A1 gene were 83.75%, 16.25% in cholesterol cholecystolithiasis patients and 81.91% and 18.09% in control group. There was no significant difference in frequencies of allele and genotype in A-204C polymorphism between two groups (Pgt;0.05). In control group and cholesterol cholecystolithiasis group， LDL-C levels in AA genotypes were lower than those in CC and CA genotype (Plt;0.05). Conclusion The results indicate that no direct association is found between CYP7A-1 gene and cholesterol cholecystolithiasis，but there is significant correlation between the polymorphism of the CYP7A-1 gene and the levels of LDL-C.
ObjectiveTo observe the effect of 5Fluorouracil(5FU) on the exocrine pancreas. MethodsThe effects of 5FU were investigated in 8 patients who had undergone pancreatoduodenectomy. The pancreatic juice was temporarily diverted to the exterior via a pancreatic duct catheter.Ten days after operation,the patients were injected intravenously with 5FU 500 mg/d for three days. The samples of pancreatic juice were taken for measurement of amylase,pH, HCO3-, Na+, K+, Cl-, Ca2+ and Mg2+.ResultsThe amylase, pH, HCO3-, Na+, K+, Cl-, Ca2+ and Mg2+ did not alteredsignificantly before and after 5FU injection.Conclusion5FU has no shortterm effect on exocrine pancreas. Therefore, improvement of acute pancreatitis cannot be achieved through inhibiting pancreatic enzymes synthesis.
Objective To investigate the inhibitory effects of RNA interference (RNAi) expression vector on the expression of survivin in pancreatic cancer cell PANC-1. Methods The protein and mRNA expressions of survivin were examined with immunofluorescence and RT-PCR. The survivin gene was cloned into the T-vector and sequenced. The RNAi expression vectors targeting survivin, named si-svv-1 and si-svv-2 respectively according to whether they harbored a mutation or no mutation, were constructed and transfected into PANC-1 cells with liposome. The expression of survivin mRNA was detected with RT-PCR. Apoptosis of PANC-1 cells was analyzed with DNA ladder and FACS. Results There was a high degree expression of survivin in PANC-1 cells. The expression of survivin was not inhibited by RNAi expression vectors si-svv-1, but inhibited about (72.43±8.04)% by si-svv-2 and the apoptosis rate of PANC-1 cells increased to （12.36±1.44）% after 72 h. Conclusion The RNAi expression vector can effectively inhibit the expression of survivin in pancreatic cancer cell PANC-1 cells and induce the apoptosis in PANC-1 cells.
Objective To investigate the frequency of variant at XmnⅠ, MspⅠ sites of apolipoprotein (Apo) AⅠ-CⅢ-AⅣ gene cluster and its relation to cholesterol cholecystolithiasis in Chinese population. Methods The restriction fragment length polymorphisms (RFLP) at Xmn Ⅰ, MspⅠ sites of ApoAⅠ-CⅢ-AⅣ gene cluster was studied by using polymerase chain reaction (PCR) in healthy subjects (control group) and patients with gallstones(gallstone group) from a population of Chinese with the Han nationality in Sichuan Province. Results The X1, M1 alleles were the major alleles in both gallstone group and control group, and X1X1, M1M homozygous genotypes were the most frequent ones. The frequencies of X1X2, X2X2 and X2 alleles in female patients of the gallstone group were significantly higher than those in female of control group (P＜0.05), and the frequencies of X1X1 and X1 alleles in control group were significantly higher than those in gallstone group (P＜0.05). The frequencies of M1M1 in male patients of the gallstone group were significantly lower than those in male of control group (P＜0.05), whereas, the frequencies of M1M2 were significantly higher in gallstone group (P＜0.05). Conclusion The polymorphism of XmnⅠ RFLP of ApoAⅠ-CⅢ-AⅣ gene cluster in female and the polymorphism of MspⅠ RFLP of ApoAⅠ-CⅢ-AⅣ gene cluster in male may be associated with cholesterol cholecystolithiasis in Chinese population.