Objective To investigate the clinical and pathological characteristics, prognosis and treatment strategies of adenocarcinoma in situ (AIS) and minimally invasive adenocarcinoma (MIA). Methods We retrospectively analyzed the clinical data of 489 patients with AIS and MIA in our hospital from January 2007 to August 2015. There were 122 males and 367 females with an average age of 26–78 (51±9) years. According to the pathological types, they were divided into the AIS group (246 patients) and the MIA group (243 patients). In the AIS group, there were 60 males and 186 females with an average age of 50±7 years. In the MIA group, there were 62 males and 181 females with an average age of 54±5 years. The clinicopathological features, surgical methods and prognosis of the two groups were compared. Results There were significant differences in age, value of carcino-embryonic antigen (CEA), nodule shape and nodule size between the AIS and MIA groups (P<0.05). AIS patients were mostly under the age of 60 years with the value of CEA in the normal range which often appeared as pure ground-glass opacity lung nodules <1 cm in diameter on the CT scan. MIA often appeared as mixed ground-glass nodules <1.5 cm in diameter, accompanied by bronchiectasis and pleural indentation. The 5-year disease-free survival rate of the AIS and MIA groups reached 100%, and there was no statistical difference in the prognosis between the two groups after subtotal lobectomy (pulmonary resection and wedge resection) and lobectomy, systematic lymph node dissection and mediastinal lymph node sampling. Conclusion The analysis of preoperative clinical and imaging features can predict the AIS and MIA and provide individualized surgery and postoperative treatment program.
Objective To study the molecular characteristics of RNA binding protein aldehyde dehydrogenase 18 family member A1 (ALDH18A1) in esophageal carcinoma cells (KYSE150 cells) and its effect on tumor growth. MethodsHuman esophageal squamous cell (KYSE150 cells) was cultured in vitro. At the same time, RNA co-immuno precipitation technology was used to study the binding of RNA and protein in the cell, and the corresponding RNA-protein complex was precipitated by the antibody of the target protein to separate and purify the captured RNA. The molecular characteristics of ALDH18A1 binding RNA were analyzed, and KyotoEncyclopedia of Genes and Genomes cluster analysis was performed for ALDH18A1 binding target genes. Results Protein immunoblotting experiments showed that the target protein was well enriched by antibodies. ALDH18A1 had extensive RNA binding activity, with significant enrichment in regions such as coding sequences, intron, and 5’untranslated region. ALDH18A1 mainly bound to the UGUAAUC motif of RNA. The cluster analysis showed that the RNA molecules bound to ALDH18A1 mainly participated in focal adhesion, central carbon metabolism in cancer, cell cycle, spliceosome, RNA transport, and ubiquitin mediated protein hydrolysis. Conclusion ALDH18A1 has the function of binding to RNA molecules and may play a role in the expression of esophageal cancer-related genes and related biological processes.
Objective To observe the effects of valsartan/ hydrochlorothiazide and valsartan on left ventricular thickness and the left ventricular diastolic function in patients with essential hypertension and left ventricular hypertrophy and impaired left ventricular diastolic function. Methods 56 patients of essential hypertension with left ventricular hypertrophy and impaired left ventricular diastolic function were randomized into two randomized double-blind groups, valsartan/hydrochlorothiazide (HCTZ) 80/12.5 mg o.d were gave to A group and valsartan 80 mg o.d were gave to B group. The dosage would be doubled in patients whose SDBP ≥ 12 kPa or SSBP ≥ 18.7 kPa after 4 weeks. Treatment lasted for 6 months. Result At the end of 6 months, valsartan/ hydrochlorothiazide and valsartan significantly reduced BP from baseline (Plt;0.01), there was significant difference in reducing BP between the two groups (Plt;0.05). Indexes of left ventricular diastolic function (IVST, LVPWT, LVMI) significantly decreased (Plt;0.01). LVEF increased significantly (Plt;0.01). There was significant difference in IVST, LVPWT, LVMI and LVEF between two groups (Plt;0.05). Conclusion Valsartan/ hydrochlorothiazide (HCTZ) can not only decrease blood pressure effectively, but also can significantly improve left ventricular hypertrophy and left ventricular diastolic function.
目的 讨论多窗技术+凝血酶封闭在CT导向下经皮肺穿刺活检中的应用价值。 方法 2009年6月－2010年3月收集分析由同一工作组连续完成的CT导向下肺穿刺活检患者共128例，其中A组58例，采用双窗技术+注射生理盐水；B组70例，采用多窗技术+注射凝血酶）。比较两组患者的诊断阳性率、气胸及肺出血发生率的差异。 结果 128例均穿刺成功，A组的穿刺诊断阳性率、气胸发生率及肺出血发生率分别为87.9%、13.8%、17.5%。B组的穿刺诊断阳性率为92.9%，气胸发生率为8.6%，肺出血发生率为5.7%。两组穿刺诊断阳性率和气胸发生率的差异无统计学意义（P＞0.05）；B组肺出血的发生率均明显低于A组，两组间肺内出血的发生率差异有统计学意义（P＜0.05）。 结论 多窗技术+凝血酶针道封闭技术能有助于减少气胸、肺出血等肺穿刺活术的并发症，具有重要的临床应用价值。
Objective To investigate the inhibitory effects and related mechanisms of NOD like receptor protein 3 (NLRP-3) inflammasome inhibitor MCC950 on oxidative stress, inflammation, and pyroptosis in human esophageal epithelial cells (HEECs). MethodsHEECs cells were passaged and divided into blank control group, acid stimulation group (stimulated 3 times a day with pH 4 acidic medium for 15 minutes each time, cultured for 48 hours), bile salt stimulation group (stimulated 3 times a day with 400 μmol/L bile salt mixture for 15 minutes each time, cultured for 48 hours), lipopolysaccharide (LPS) group (stimulated with 10 μL of 100 ng/mL LPS for 48 hours), MCC950 group (stimulated HEECs cells with 10 μL of 7.5 ng/mL MCC950 for 4 hours, then stimulated with acid, bile hydrochloric acid, and LPS for 48 hours), and N-acetyl-L-cysteine (NAC) group (stimulated HEECs cells with 1 mmol/L NAC for 4 hours, then stimulated with acid, bile hydrochloric acid, and LPS and incubated for 48 hours). Three culture dishes were used in each group to detect the mRNA and protein expression levels of oxidative protein/antioxidant protein [Nox-4 (NADPH oxidase 4), nuclearfactor erythroidderived 2-like 2 (Nrf-2), heme oxygenase-1 (HO-1)], NLRP-3 signaling pathway [NLRP3/caspase-1/intereukin-1β (IL-1β)/intereukin-18 (IL-18)], and cell apoptosis pathway [caspase-4/caspase-5/GSDMD] using real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting experiments. Cell apoptosis were observed through Hoechst 33342 staining. ResultsMCC950 intervention (average optical density: 0.023) and NAC intervention (average optical density: 0.031) effectively inhibited HEECs apoptosis induced by acid (average optical density: 0.042), bile salt (average optical density: 0.047), and LPS (average optical density: 0.054). The results of RT-PCR and Western blotting experiments showed that MCC950 intervention and NAC intervention significantly inhibited the high expression of Nox-4 mRNA (MCC950:1.68±0.18, NAC: 1.62±0.17) and protein in HEECs cells induced by acid (1.00±0.05), bile salt (3.07±0.25), and LPS (3.52±0.37); And significantly upregulated the mRNA and protein expression levels of antioxidant proteins Nrf-2 (MCC950: 0.72±0.12, NAC: 0.57±0.12) and HO-1 (MCC950: 0.74±0.12, NAC: 0.57±0.12). MCC950 intervention and antioxidant NAC intervention effectively inhibited the mRNA and protein expression levels of acid stimulated, bile salt stimulated, and LPS induced HEECs cell NLRP-3 (MCC950 intervention: 1.58±0.06, NAC intervention: 1.47±0.09), ASC (MCC950 intervention: 1.56±0.09, NAC intervention: 1.93±0.17), caspase-1 (MCC950 intervention: 1.64± 0.13, NAC intervention: 1.96±0.20), IL-1β (MCC950 intervention: 1.66±0.18, NAC intervention: 1.82±0.20), IL-18 (MCC950 intervention: 1.58±0.13, NAC intervention: 1.84±0.16) and other indicators. MCC950 intervention and antioxidant NAC intervention effectively inhibited the mRNA and protein expression levels of apoptosis pathway markers such as caspase-4 (MCC950 intervention:1.51±0.03, NAC intervention: 1.61±0.12), caspase-5(MCC950 intervention: 1.38±0.13, NAC intervention: 1.64±0.21), and GSDMD (MCC950 intervention: 1.41±0.04, NAC intervention: 1.54±0.10) induced by acid stimulation, bile salt stimulation, and LPS in HEECs cells. ConclusionAcid, bile salts, and LPS can all induce the overexpression of oxidative stress markers in HEECs, reduce the expression of antioxidant proteins, and activate the NLRP3 inflammasome signaling pathway and cell pyroptosis pathway, promoting cellular inflammatory damage, but MCC950 has a protective effect.