Objective To investigate the expression of RNA editase ADAR1 in the lymphocytes in rats’ spleen with liver transplantation rejection. Methods Thirty SD rats and 75 Wistar rats were included. Fifteen livers from Wistar rats were transplanted to 15 Wistar rats (isograft group), 30 livers from SD rats were transplanted to 30 Wistar rats (allograft group and allograft+FK506 group), and 15 of them were then intramuscularly injected with FK506, 2 mg/(kg·d), the other 15 Wistar rats were only operated similarly to the other rats without any liver transplantation (control group). Five rats were killed and their splenetic tissues were collected on day 3, day 5, and day 7, respectively. The expression of ADAR1 mRNA in lymphocytes of the spleen in acute rejection was detected by RT-PCR. Results Different performance of pathology was observed in all the liver and spleen tissues from the transplanted rats over time, especially in allograft group. The expression of ADAR1 mRNA in the allograft group was significant higher than that of isograft and allograft+FK506 groups （P＜0.001）, especially on the 5th day. Conclusion There was a significant positive correlation between expression of ADAR1 and the severity of acute rejection, but the mechanism by which ADAR1 affected the acute rejection is unknown and needs to be further studied. FK506 may inhibit the expression of ADAR1 and remarkably reduce the severity of acute rejection.
【Abstract】ObjectiveTo investigate the effect of RNA editing enzyme ADAR1 on the function of lymphocyte immune by transferring mouse lymphocytes with plasmid of sense siRNA and by suppressing the expression of ADAR1. MethodsThe cell strains of human hepatic cellular carcinoma （HCC） were frozen and thawed repeatedly to prepare for tumor soluble antigen. The isolated mouse lymphocytes, which were transferred with antisense siRNA plasmid of ADAR1 and were sensitized with soluble tumor antigen were used as the study group; those which were not transferred but were sensitized were used as the control group. The 3HTdR adulteration experiment was used to test the sensitivity of lymphocytes. The effect of ADAR1 on lymphocyte immunity was detected by lymphocytotoxicity tests. ResultsThe observation of the isolated lymphocytes implied that the growth cycle of lymphocyte was 10-14 days. The 3HTdR adulteration experiment showed the result was optimal. The number of HCCs decreased significantly for both of the groups compared with those in the blank holes, but the amplitude was much larger in the control group. The expression of ADAR1 in lymphocytes of the study group was significantly lower than that of the control group, which demonstrated that the RNA plasmid of ADAR1 suppressed the expression of ADAR1 in sensitized lymphocytes and the suppressing rate of the control group (87.47±4.62)% was significantly higher than that of study group （53.19±3.95）%. The function of lymphocytes killing targetcells in the study group was significantly inferior to that of control group (P＜0.05). ConclusionRNA editing enzyme ADAR1 may play an important role in mouse cellullar immunologic response and it is possible to attenuate the cellimmune response by depressing the expression of ADAR1.