Objective To evaluate early results of valved bovine jugular vein patch for reconstruction of the right ventricular outflow tract (RVOT).?Methods From May 2009 to March 2010, a total of 60 patients with complex congenital heart diseases underwent reconstruction of RVOT with valved bovine jugular vein patch in Wuhan Asia Heart Hospital. There were 42 males and 18 females with their mean age of 6.2±8.9 years (ranging from 5 months to 33 years) and mean body weight of 27.5±24.0 kg, and 34 patients were less than 1 year. Preoperative clinical diagnosis included tetralogy of Fallot (n=38) and double outlet of right ventricle with pulmonary stenosis (n=22). All the patients underwent one-stage surgical repair. Before operation, 4 patients underwent catheter intervention for their major aortopulmonary collaterals. The diameters of pulmonary arterial ring of all the patients were 2 standard deviation less than normal range, and trans-annular patch was chosen for RVOT reconstruction. All the patients were postoperatively followed up for 18 to 26 months (mean 21.2±4.6 months).?Results There was no in-hospital death. And no second surgical intervention was needed for conspicuous RVOT stenosis or pulmonary regurgitation. Three patients needed reintubation for lung edema after extubation as a result of major aortopulmonary collaterals. Four patients underwent reexploration for postoperative bleeding. And all the other patients were discharged uneventfully. Mean cardiopulmonary bypass time was 84.0±22.0 min, and mean aortic cross-clamping time was 42.0±12.0 min. Mean RVOT gradient right after surgery was 18.0±4.5 mm Hg, which was not statistically different from mean RVOT gradient of 19.2±5.4 mm Hg measured by transthoracic echocardiography at their last postoperative follow-up(P>0.05). The degree of pulmonary regurgitation right after surgery was trivial in 32 patients(1+), mild in 28 patients(2+), which were not statistically different from the degree of pulmonary regurgitation at their last postoperative follow-up: trivial in 28 patients (1+), mild in 27 patients(2+), and moderate in 5 patients(3+). Calcification was not observed on the valved bovine jugular vein patch and valve cusp, and the valve cusp motioned well. No thrombosis or endocarditis was observed on the valved bovine jugular vein.?Conclusions For patients with tetralogy of Fallot or double outlet of right ventricle (DORV) and pulmonary stenosis, valved bovine jugular vein patch is a good choice for trans-annular reconstruction of RVOT. There is no severe postoperative complication related to bovine jugular vein, the RVOT pressure gradient does not increase significantly, and anti-regurgitation result is satisfactory in short-term follow-up. Further follow-up is required to evaluate its long-term outcome.