west china medical publishers
Author
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Author "ZHU Hongyu." 1 results
  • The Effect of Ischemic Preconditioning on Myocardial Ischemic Reperfusion of Elderly Rats

    Objective To investigate the effect of ischemic preconditioning(IPC) on myocardial ischemic reperfusion injury(I/R) of elderly rats. Methods Fiftysix Wistar rats, of which there were 28 aged from 21-23months(elderly rat) and 28 aged from 4-5months(young rat), were used to build isolated heart perfusion Langendorff model. The rats were divided into 7 groups with random number table(8 in each group): adult control group, adult I/R group, adult IPC group, elderly control group, elderly I/R group, elderly IPC group and elderly enhanced IPC group. The control group underwent a 90-min perfusion without any intervention; the I/R group underwent a 30-min equilibration period, then a 30-min ischemia and a 30-min reperfusion; the IPC group underwent a 10-min equilibration period, then a 5-min ischemia for twice and a 5-min reperfusion, after that a 30-min ischemia and [CM(158-3mm]a 30-min reperfusion; the enhanced IPC group underwent a 10-min equilibration period, then a 5-min ischemia for 4-times and a 5-min reperfusion, after that a 30-min ischemia and a 30-min reperfusion. The recovery rates of cardiac output(CO), left ventricular developed pressure (LVDP), the maximum rising and descending rate of left ventricular pressure (±dp/dtmax) after a 30-min reperfusion were compared among groups. The activity of creatine kinase (CK) in coronary outflow, the level of malonyldialdehyde (MDA) and superoxide dismutase (SOD) before ischemia and after a 30min reperfusion were detected. The myocardial infarction areas were compared among groups. Results After a 30min reperfusion, compared with adult I/R group, in adult IPC group CK reduced significantly(89.48±18.72 U/L vs. 115.76±16.72 U/L,q=6.061,Plt;0.01),the level of MDA decreased significantly(9.53±3.44 nmol/ml vs. 16.84±2.29 nmol/ml,q=7.732,Plt;0.01),the level of SOD increased significantly(584.7±122.62 U/ml vs. 429.46±85.24 U/ml,q=4.754,Plt;0.01),the recovery rates of CO,LVDP,+dp/dtmax and -dp/dtmax increased ignificantly(78.69%±9.68% vs. 65.10%±8.63%,83.61%±8.46% vs. 67.23±8.68%,81.68±8.68% vs. 67.89%±6.89%,89.79%±7.78% vs. 66.79%±8.46%,Plt;0.01), the myocardial infarction areas reduced significantly (5.25%±4.33% vs. 14.75%±8.02%,q=7.458,Plt;0.01)。There was no statistical significance between elderly IPC group and elderly I/R group in the above indexes(Pgt;0.05).However, There was statistical significances between elderly enhanced IPC group and I/R group. CK reduced significantly (88.60±28.32 U/L vs. 105.76±9.64 U/L,q=5.620,Plt;0.01),the level of MDA decreased significantly(8.38±3.36 nmol/ml vs. 16.80±3.06 nmol/ml,q=7.500,Plt;0.01),the level of SOD increased significantly(558.87±78.66 U/ml vs. 433.75±86.65 U/ml,q=7.335,Plt;0.01),the recovery rates of CO,LVDP,+dp/dtmax and -dp/dtmax increased significantly (77.99%±10.02% vs. 66.26%±9.78%,85.59%±6.67% vs. 73.90%±6.66%,83.87%±9.98% vs. 68.90%±8.68%,86.01%±766% vs. 70.39%±7.98%,Plt;0.01), the myocardial infarction areas reduced significantly (795%±6.32% vs. 1568%±10.36%,q=8.680, Plt;0.01). 〖WTHZ〗Conclusion The protective effect of IPC on I/R elderly rat hearts has weakened. The enhanced IPC is able to regain the protective effect of IPC on elderly rat hearts.

    Release date:2016-08-30 06:06 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content