ObjectiveTo evaluate the safety and efficacy of single-layer seromuscular suture in ileocolonic anastomosis. MethodsFifty patients who needed ileocolonic anastomosis sugery were randomly divided into seromuscular anastomosis group (n=25) and two-layer anastomosis group (n=25). Intraoperative blood loss, perative time, postopreative complications, bowel function recovery time, and postoperative hospital stay time were recorded in each group. ResultsBlood loss, anastomotic leak, first passing gas time, first defecation time, and postoperative hospital stay time were (11.00±3.24) ml vs (10.28±2.99) ml (Pgt;0.05), 4.0% vs 8.0% (Pgt;0.05), (3.72±1.59) d vs (4.72±1.59) d (Pgt;0.05), (5.08±3.36) d vs (6.08±3.36) d (Pgt;0.05), and (8.47±2.32) d vs (10.26±2.61) d (Pgt;0.05), all the indexes above between the two groups had no significant difference. While a significant differnece in operative time was found between them 〔(11.74±2.02) min vs (16.44±2.06) min), Plt;0.01〕. ConclusionsSingle-layer seromuscular suture technique is safe and effective in ileocolonic anastomosis.
Objective To understand the research status of social network analysis methods in the medicine and health field, help medical scientific research managers quickly understand the publication situation and research hotspots of the methods, and provide references for them to use social network analysis methods to enter deeper research. Methods PubMed, Web of Science, Springer Link, ScienceDirect, China National Knowledge Infrastructure, Wanfang and VIP databases were searched for related literature on social network analysis methods in the medical and health field from the establishment of databases to April 2022. Bibliometric analysis was used to analyze the included articles. Results A total of 432 articles were included, with 424 in Chinese and 8 in English. The included articles were published between 1993 and 2020, involving 154 journals and 913 key words. The number of documents increased rapidly at first, and then entered a stable stage. The hot research directions were the spread and prevention of diseases and the power of social support networks. Conclusions Although the number of applications of social network analysis methods in the medical and health field has increased year by year and the application flexibility has increased, the application depth is still lacking. Scientific researchers should dig deep into the research direction, combine theory with practice, and focus on innovation.
Objective To investigate the effect of S-adenosylmethionine (SAM) on mitochondrial injury that was induced by ischemia-reperfusion in rat liver. Methods Fifty-four rats were randomly divided equally into 3 groups: control group, ischemia-reperfusion group (I/R group), and SAM-treated group (SAM group). Hepatic ischemia had been only lasted for 30 min by obstructing the blood stream of hepatic portal vena (the portal vena was only separated but not obstructed in control group). The rats of SAM group received SAM intraperitoneally 2 h prior to ischemia. Blood samples of each group were collected from the inferior cava vena at 0, 1 and 6 h after reperfusion and the serum levels of AST and ALT were detected. Mitochondrial super oxidedismutase (SOD), malondialdehyde (MDA), adenosine triphosphate (ATP) and energy charge (EC) in samples of liver tissue were detected, and the mitochondrial ultrastructure was observed with electronmicroscope. Results The serum levels of AST, ALT and mitochondrial MDA at 0, 1 and 6 h after reperfusion in the I/R group were significantly higher than those in the control group, whereas the levels of mitochondrial SOD, ATP and EC were significantly lower than those in the control group (P＜0.01). Except the value of 0 h, when it comes to SAM group, the levels of AST, ALT and mitochondrial MDA were significantly lower (P＜0.05) and the levels of mitochondrial SOD, ATP and EC were significantly higher (P＜0.05, P＜0.01) than those in the I/R group, respectively. The mitochondrial ultrastructure was injured obviously in I/R group when compared with that in control group. The number of mitochondria decreased and the mitochondria swelled, making the crista became obscure and the density of matrix became lower. The above changes in SAM group were less obvious when compared with those in I/R group. Conclusion SAM may protect mitochondrion against hepatic ischemia injury, since it may prevent mitochondrial lipid peroxidation, increase ATP, and eventually improve energy metabolism after ischemia-reperfusion.
Objective To explore the impact of community healthcare workers’ (CHWs) knowledge, attitude and practice (KAP) on the influenza vaccination among elderly people. Methods By means of simple random sampling, 1 residential quarter of each communities, 2 communities of each districts, 5 districts of Chengdu city were randomly selected, and the elderly equal to or more than 60-year-old were on-site investigated. Meanwhile, the questionnaire survey was conducted among healthcare workers in the selected communities. Results There were 4 KAP factors played a positive role in influenza vaccination among elderly people: CHWs’ affirmation of the effectiveness of influenza vaccine, explicitly knowing the focus groups for influenza vaccination, recommendation of vaccination in flu season when the elderly visits, and participation in flu-related education activities. When the accuracy rate of each factor got improved by 1%, the influenza vaccination rate would improve by 2.747%, 1.299%, 0.864%, 0.602%, respectively. Conclusion The knowledge, attitude and practice of HCWs have impacts on the influenza vaccination rates of elderly people. They are significant to improve the influenza vaccination rates of the elderly.
Objective To analyze the surgical techniques for the procurement and back-table surgery of the graft in living-related small bowel transplantation. Methods Eligible donor was chosen according to the donor selection criteria of living-related small bowel transplantation, and preoperative plan was designed. A segment of ileum of 120 cm was procured 20 cm proximal to the ileocecal valve which was preserved in the donor. The techniques for the procurement and back-table surgery of the graft were summarized, which included measurement of entire small bowel length from Treitz to ileocecal valve, palpation and transillumination to identify the distal branch of the superior mesenteric artery， and transient blockage of isolated blood vessels with vascular clamps in order to observe the influence on the blood circulation of graft and residual ileum. The detailed manipulation techniques in processing the graft blood vessels were discussed. Results The operations were successful both on the donor and the recipient. The functions of implanted segment of bowel were well. The donor had no other complications, such as mesenteric thrombi and anastomosis leakage of intestine, except for transient moderate diarrhea. She was discharged 14 days after operation. In the next 8 months of following-up, the donor has not experienced significant alteration in bowel habits or weight loss. Now she is in good appetite, without any changes in the habit and amount on diet. No changes have been found in lifestyle, work habits, or psychosocial conditions after the small bowel donation. Conclusion The procurement of a segment of ileum as graft and preservation of 20 cm proximal to the donor ileocecal valve may be ideal protocol. Using a standardized technique with attention presents little recent or long-term risks for the donor and brings satisfied effect for the recipient.