Objectives To investigate the expressions and significance of E2F1, ID1, and Bax protein in gallbladder adenocarcinoma tissues. MethodsThe expressions of E2F1, ID1, and Bax protein in 70 cases of gallbladder adenocarcinoma, 20 cases of high level intraepithelial neoplasia, 30 cases of low level intraepithelial neoplasia, and 20 cases of cholecystitis tissues were tested by using immunohistochemical method. ResultsThe positive expression rates of E2F1, ID1, and Bax protein in gallbladder adenocarcinoma was 84.3%, 70.0%, and 25.7%, respectively; the positive expression rates in high level intraepithelial neoplasia was 75.0%, 65.0%, and 55.0%, respectively; the positive expression rates in low level intraepithelial neoplasia was 16.7%, 23.3%, and 56.7%, respectively; and the positive expression rates in cholecystitis tissues was 10.0%, 20.0%, and 75%, respectively.The positive expression rates of E2F1 and ID1 protein in gallbladder adenocarcinoma were significantly higher than those intraepithelial neoplasia and cholecystitis tissues (P < 0.05), but the positive expression rate of Bax protein in gallbladder adenocarcinoma was lower (P < 0.05).The expressions of E2F1 and ID1 protein were significantly correlated with clinical Nevin staging of gallbladder adenocarcinoma (P < 0.05), but not correlated with the gallbladder adenocarcinoma differentiation degree (P > 0.05).The expression of Bax protein was related to the gallbladder adenocarcinoma differentiation degree (P < 0.05), but not correlated with clinical Nevin staging (P > 0.05).The expression of E2F1 protein was negatively correlated with expression of Bax protein (r=-0.375, P < 0.05), ID1 protein expression has nothing to do with the protein expression of Bax protein (P > 0.05).The expression of E2F1 protein was positively correlated with ID1 protein (r=7.031, P < 0.05). ConclusionsThe E2F1, ID1, and Bax may play an important role in the generation and development of the gallbladder adenocarcinoma.The combined detection of E2F1, ID1, and Bax have important guiding significance for auxiliary diagnosis and clinical staging of gallbladder adenocarcinoma.
ObjectiveTo explore the expression of Cryab in human colorectal cancer tissue and its clinical significances. MethodsTen pairs of colorectal cancer specimens of early recurrence and late recurrence in the First Hospital of Lanzhou University were collected, the Cryab mRNA expression was detected by RT-PCR. The colorectal cancer tissues and corresponding adjacent tissues of 100 patients underwent radical resection from January 2005 to December 2008 in the Donggang Branch of The First Hospital and The First Hospital of Lanzhou University were collected, the expression of Cryab protein in the colorectal cancer tissues and corresponding adjacent tissues of 100 patients with colorectal cancer were detected by immunohistochemistry SP technique. The relation between the expression of Cryab protein and the age, gender, number of tumors, tumor size, TNM stage, degree of differentiation, lymph node metastasis, or depth of invasion of the patients was analyzed. The influence of Cryab expression on prognosis of patients with colorectal cancer was evaluated by univariate and multivariate analysis. Results①The expression of Cryab mRNA in the colorectal cancer tissues with early recurrence was significantly higher than that in the colorectal cancer tissues with late recurrence (P < 0.05).②The expression of Cryab protein in the colorectal cancer tissues was significantly higher than that in the corresponding adjacent tissues (P < 0.05).③The over-expression of Cryab protein in the colorectal cancer tissues was correlated with the tumor size, degree of differentiation, lymph node metastasis, or TNM stages (P < 0.05), but not correlated with the age, sex, and number of tumors (P > 0.05).④The lymph node metastasis and expression of Cryab protein were the independent risk factors for the prognosis of patients with colorectal cancer.⑤The overall survival and disease free survival of patients with high-expression of Cryab protein were significantly lower than those in the patients with lower-expression of Cryab protein. ConclusionsThe over-expression of Cryab is closely related to the invasion and progression of colorectal cancer, they might be together involved in the progression of colorectal cancer. Cryab might be a good marker for monitoring the malignant degree and the prognosis of colorectal cancer.
ObjectiveTo investigate the relationship between the changes of nerve cells in sphincter of Oddi and acute pancreatitis. MethodsThe rabbit models of acute pancreatitis were prepared by using sodium taurocholate perfusion. Immunohistochemical method was used to detect the expressions of nitric oxide synthase (NOS) and vasoactive intestinal peptide (VIP) in neurons of the sphincter of Oddi. ResultsIn the control group, (45.83±2.17)% of myenteric neurons were NOS-positive, (52.46±2.47)% of myenteric neurons were VIP positive, and (22.73±1.95)% of myenteric neurons were NOS and VIP double positive. In contrast, (11.26±0.93)% of myenteric neurons were NOS-positive and (28.62±2.83)% of myenteric neurons were VIP positive in SAP group, which were significantly less than those of control group (P < 0.01). ConclusionsThe sphincter of Oddi of normal rabbits is rich in VIP and NOS positive neurons. The significant reduction of NOS-positive and VIP-positive neurons when SAP, which may be the reason of decreased the activities of the sphincter of Oddi.