Objective To provide basis for cl inical appl ication of ANKYLOS dental implants by following up alveolar bone status of 318 pieces of restored ANKYLOS dental implants. Methods Between February 2008 and August 2009, 170 patients with dentition defect underwent placement of ANKYLOS dental implants (318 pieces). There were 74 males (133 pieces) and 96 females (185 pieces) with an average age of 43.8 years (range, 23-68 years). After operation, the periapicalX-ray films were taken to observe osseointegration around the neck of implant, alveolar bone resorption, and survival ofimplants. Results All patients were followed up at 6, 12, and 24 months after operation. There were 9 failure implants witha total dental implants survival rate of 97.17% (309/318): 3 at 6 months, 4 at 6-12 months, and 2 at 12-24 months, showing no significant difference in dental implants survival rate among 3 time points (χ2=0.470 3, P=0.492 8). New bone formed around the neck of implant in 4 cases at 6 months and in 31 cases at 12 months; at 6, 12, and 24 months, the bone increase was (0.392 7 ± 0.217 4), (0.633 5 ± 0.202 1), and (0.709 0 ± 0.199 1) mm, respectively, showing significant differences among 3 time points (P lt; 0.05). At 6, 12, and 24 months after operation, the bone loss of other patients was (0.392 7 ± 0.217 4), (0.716 7 ± 0.220 3), and (0.723 2 ± 0.215 4) mm, respectively, showing significant differences among 3 time points (P lt; 0.05). Conclusion After restoration with ANKYLOS dental implant, alveolar bone status is good and the implant success rate is high during short-term follow-up. But further observation and study are required for long-term effectivness.
This study is to investigate the inhibitory effect of different concentrations of zoledronic acid on the activity of osteoclasts, to obtain characteristics on inhibitory effect and to find the lowest effective concentration of zoledronic acid. Marrow cells of C57 mice (6 weeks) were cultured in vitro. Osteoclasts were induced by single nuclear cells. According to the concentration of zoledronic acid, we set up the experimental group with five different concentrations, i.e. 1×10–8 mol/L, 1×10–7 mol/L, 1×10–6 mol/L, 1×10–5 mol/L, and 1×10–4 mol/L. The control group did not contain any bisphosphonate. By tartrate resistant acid phosphatase staining, the number of multinuclear cells, cells through the filter and bone resorption lacune were counted. Five days after the cultivation, the number of multinuclear cells in the experimental group decreased with the increase of concentration of zoledronic acid. Inhibition on the formation of osteoclasts in vitro was effective at 1×10–6 mol/L. At the concentration of 1×10–5 mol/L, the effect of inhibition on migration of osteoclast and bone resorption was more obvious. The effect was further enhanced at concentration of 1×10–4 mol/L. However, the concentration and inhibition curves were gradually mild. The inhibitory effect on different concentrations of zoledronic acid on the activity of osteoclasts was different. The inhibition effect was obvious at 1×10–6 mol/L. We should pay attention to administrate appropriate concentration of zoledronic acid in the clinical applications.
Objective To investigate the effect ofstaphylococcal lipoteichoic acid (LTA-sa) on RAW264.7 cells differentiation into osteoclasts. Methods RAW264.7 cells were cultured with LTA-sa of 100 ng/mL (group A), LTA-sa of 200 ng/mL (group B), LTA-sa of 400 ng/mL (group C), receptor activator of nuclear factor κB (NF-κB) ligand (RANKL) of 100 ng/mL as positive control (group D), and equal volume of PBS as blank control (group E) respectively for 5 days. And then, tartrate resistant acid phosphatase staining (TRAP) was used to detect the formation of osteoclast-like cells, Image-Pro Plus 6.0 software to measure the areas of bone resorption pits in Corning Osteo Assay Surface (COAS) wells, and MTT assay to observe the proliferation activity of RAW264.7 cells in group A, B, C, and E. Results After cultured for 5 days, the formation of osteoclast-like cells and bone resorption pits were observed in all groups. The number of osteoclast-like cells and the area of bone resorption pits in groups A, B, C, and D were more than those in group E. And with the increased concentration of LTA-sa, the indexes in groups A, B, and C increased gradually, but were lower than those in group D, and differences were significant between groups (P<0.05). At 5 days after culture, there was no significant difference in absorbance value among the experimental groups (groups A, B, C, and E) (P>0.05). Conclusion LTA-sa has promoting effect on RAW264.7 cells differentiation into osteoclasts.