Objective To evaluate the association between methylene tetrahydrofolate reductase (MTHFR) A1298C polymorphism and breast cancer in the Asain population. Methods PubMed, EMBASE, Web of science, Chinese Biomedical Literature Database, Chinese Science and Technology Academic Periodical Database, Chinese Journal Full-Text Database, and Wanfang Database were searched to identify relevant articles that investigated the association of MTHFR A1298C polymorphism with breast cancer, the articles published time was from inception of database to March 2017. A meta-analysis was performed by using the Stata 12.0 software. Results A meta-analysis of 19 case-control studies was performed including 7 454 cases of breast cancer and 8 255 controls. The results of meta-analysis suggested that there was no significant association between MTHFR A1298C polymorphism and breast cancer for the Asain population under five genetic models 〔AA vs AC: OR=0.97, 95% CI was (0.91, 1.04), P=0.374; AC vs CC: OR=0.87, 95% CI was (0.75, 1.01）, P=0.060; AA vs CC: OR=0.87, 95% CI was (0.76, 1.01), P=0.061; AA+AC vs CC: OR=0.88, 95% CI was (0.76, 1.01), P=0.061; AC+CC vs AA: OR=0.99, 95% CI was (0.93, 1.06), P=0.737〕. Conclusion MTHFR A1298C polymorphism is not associated with breast cancer in the Asain population.