west china medical publishers
Keyword
  • Title
  • Author
  • Keyword
  • Abstract
Advance search
Advance search

Search

find Keyword "p16基因" 5 results
  • Deletion and Mutation of p16/mts1 Gene in Human Gastric Cancer of Different Periods

    ObjectiveTo determine the frequency and type of p16 gene homozygous deletion and mutation with the progression of human gastric carcinogenesis.MethodsPCR (polymerase chain reaction),SSCP (single strand conformation polymorphism) and DNA sequencing were performed on 50 operative tissues, which included 7 early gastric cancer and 43 advanced gastric cancer. In this group there were 36 male and 14 female patients aged 35 to 68,which included 30 welldifferetiated adenocarcinoma and 20 poordifferentiated carcinoma; 19 patients with lymph node metastases.ResultsTotal mutation frequency of p16 gene was 8.00%(4/50), including 14.29%(1/7) of early cancer and 7.00%(3/43) of advanced cancer,there was no significant difference between early and advanced cancer (P>0.05). In addition, p16 gene mutation was not related to site,location,grade of tumor differentiation and lymph node metastases, respectively. Total deletion frequency of p16 gene was 16.00%(8/50), of which 18.60%(8/43) was detected in advanced cancer, but no cases in early cancers. There was a significant difference between early and advanced cancer (P<0.05), and frequency p16 gene homozygous deletion was related to grade of tumor differentiation and lymph node metastases, respectively. Direct DNA sequencing displayed that 4 point mutations detected in 99,125,433,441 base, there were 3 missense mutation and 1 samesense mutation, no deletion or insertion was found. Conclusionp16 gene mutation is an early event in gastric carcinogenesis,it helps early diagnosis of human gastric carcinoma. p16 gene homozygous deletion is a late event in gastric carcinogenesis,it might relate to metastases and recurrence of gastric cancer.

    Release date:2016-08-28 05:12 Export PDF Favorites Scan
  • STUDY ON THE EXPRESSION OF p16 AND Rb IN THYROID CARCINOMA

    【Abstract】Objective To demonstrate the role of p16 protein and Rb protein in the formation and progression and their relationship in thyroid carcinoma. Methods p16 and Rb from 46 patients with thyroid cancer were detected by SP immunohistochemistry. Results In thyroid carcinoma,the p16 positive rate was decreased with the decline of differentiation. There was an inverse correlation between the rates of p16 positive expression and involvement of lymph node,clinical stage and prognosis index.The Rb protein was not present in any cases as a nucleus staining. Conclusion The p16 gene is likely to play an important role in the genesis and malignant progression of thyroid carcinoma,while the Rb gene is not .

    Release date:2016-08-28 05:30 Export PDF Favorites Scan
  • 外源性p16基因对食管癌患者癌细胞系的生长抑制作用

    目的 观察外源性p16基因质粒导入食管癌患者食管鳞癌细胞系Ec109后的表达及其对Ec109细胞增殖生长状况的影响。方法 根据转染质粒的不同或是否行p16 cDNA质粒转染分为三组,每组分别为5个样本。PcDNA3-P16转染组(P16转染组):采用脂质体(Lipofectamine)为载体,将PcDNA3-P16表达质粒导入Ec109细胞;空载体转染组:用PcDNA3-neo空载体以上述相同方法转染,作空白对照;未转染组:未用PcDNA3-P16质粒转染,作阴性对照。应用Northern斑点杂交、免疫组织化学、免疫荧光检测转化细胞的P16蛋白变化;流式细胞仪分析细胞周期变化,DNA片段化电泳检测细胞凋亡。结果 P16转染组外源性p16基因在Ec109细胞中表达后,细胞生长速度减慢,克隆形成能力下降,瞬时表达时有细胞凋亡发生,稳定表达后细胞存在G1期阻滞;空载体转染组和未转染组均未观察到以上结果。结论 脂质体介导的外源性p16基因转染对Ec109细胞有生长抑制作用,p16基因瞬时表达可诱导细胞凋亡。

    Release date:2016-08-30 06:34 Export PDF Favorites Scan
  • 食管癌p16基因变异分析

    目的 为了探讨p16基因与食管癌发生、发展的关系.方法 应用聚合酶链反应、单链构象多态分析(PCR-SSCP)方法检测30例食管癌中p16基因的纯合性缺失和突变情况,并分析p16基因变异与食管癌的病理关系.结果 30例食管癌患者中4例为p16基因纯合性缺失,2例存在突变.6例阳性样品与其它样品在年龄、饮酒史方面差异有显著性(P<0.01),与淋巴结转移、病理分期有差异(P<0.05);而与性别、肿瘤长度、肿瘤部位、病理类型无差异(P>0.05).结论 抑癌基因p16在食管癌中存在基因变异,其在食管癌的发生、发展中起重要作用.

    Release date:2016-08-30 06:35 Export PDF Favorites Scan
  • Role of Methylation of p16 Gene in Breast Cancer

    目的研究p16基因甲基化在乳腺癌中的分布情况,并探讨其与乳腺癌发生、发展的关系。方法采用甲基化特异的PCR技术检测四川省肿瘤医院乳腺科2008年3~9月期间收治的38例原发性乳腺癌组织及距离肿瘤>5 cm以远的正常乳腺组织中p16基因启动子区CpG岛甲基化频率。结果乳腺癌组织中p16基因启动子区CpG岛甲基化频率〔31.6%(12/38)〕明显高于其正常乳腺组织〔5.3%(2/38)〕,差异有统计学意义(P=0.003)。有淋巴结转移的乳腺癌患者中p16基因甲基化频率〔45.5%(10/22)〕高于无淋巴结转移患者〔12.5%(2/16)〕,差异有统计学意义(P=0.044)。结论p16基因启动子区CpG岛甲基化可能在乳腺癌发生、发展过程中起着重要作用。

    Release date:2016-09-08 10:40 Export PDF Favorites Scan
1 pages Previous 1 Next

Format

Content