Objective To investigate the role of chemokine receptor CXCR7 in the development and progression of pancreatic carcinoma. Methods The short hairpin RNA (shRNA) targeting CXCR7 was designed and delivered into AsPC-1 pancreatic carcinoma cells to knock down CXCR7 expression. The cell proliferation, cell cycle, and apoptosis after CXCR7 knockdown was determined by MTT and flow cytometry, respectively. The invasive ability of pancreatic carcinoma cells was evaluated by using the Transwell system. Results Compared with the blank control group (BC group), transfection of AsPC-1 cells with CXCR7-shRNA resulted in a significantly decreased expression of CXCR7 at both mRNA and protein levels (P<0.05), and the ability of proliferation and invasion significantly decreased (P<0.05). Knockdown of CXCR7 also significantly increase apoptosis (P<0.05), induce cell cycle arrest at G0/G1 phase (P<0.05). Conclusions Taken together, the present study showes that the knockdown of CXCR7 expression may play an important role in pancreatic carcinoma development, invasion, and metastasis, CXCR7 may be a potential therapeutic target for the treatment of pancreatic carcinoma.
ObjectiveTo explore the correlation between multiple clinicopathologic factors and perineural invasion in patients with pancreatic carcinoma.MethodsThe clinical data of 90 patients with pancreatic carcinoma in the Department of General Surgery of Shanghai Ninth People’s Hospital of Shanghai JiaoTong University School of Medicine from January 2012 to January 2020 were retrospectively collected. We collected clinical data such as patient medical records, surgical records, nursing records, examination reports, pathological reports, and studied the parameters that affect the occurrence of perineural invasion in patients with pancreatic carcinoma.ResultsAmong 90 patients with pancreatic carcinoma, 47 cases had perineural invasion and 43 cases had no perineural invasion. The univariate analysis results showed that perineural invasion was associated with abdominal pain and low back pain, levels of fasting blood glucose, glycosylated hemoglobin, CA19-9, carcinoembryonic antigen (CEA), and bilirubin, as well as tumor location, TNM stage, differentiation degree, lymph node metastasis, vascular cancer thrombus, peripheral invasion, and expression of p53 (P<0.05). Binary logistic regression analysis showed that abdominal or lower back pain, increased fasting blood glucose, tumor with invasion of surrounding tissues, and p53-positive expression were the independent risk factors for perineural invasion of pancreatic carcinoma (P<0.05).ConclusionPatients with pancreatic carcinoma have abdominal or lower back pain, elevated fasting blood glucose before surgery, tumor with surrounding tissue invasion, and p53-positive expression are independent risk factors for perineural invasion, which should be paid attention to.