Interleukin-8 antagonist down regulates the adhesion and migration of retinal vascular endothelial cells by inhibiting the production of reactive oxygen species_Chinese Journal of Ocular Fundus Diseases

Authors：

Wang Linni , Cao Jingjing , Xing Dongjun , Yu Rongguo , Hu Liying , Yang Yang , Li Chang , Li Zhiqing , Li Hui , Hong Yaru ,  Dong Lijie

Tianjin Medical University Eye Hospital, Eye Institute and School of Optometry, Tianjin Branch of National Clinical Research Center for Ocular Disease, Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin 300384, China;

Corresponding author：

Dong Lijie, Email: aitaomubang@126.com

Keywords：

Retinal vascular endothelial cells; Interleukin-8; Migration; Adhesion; Reactive Oxygen species; Cell experiment

DOI：

10.3760/cma.j.cn511434-20220315-00148

Video：

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Objective To observe the effect of interleukin-8 (IL-8) on the adhesion and migration of retinal vascular endothelial cells (RCEC). Methods A cell experiment. Human RCEC (hRCEC) was divided into normal control group (N group), advanced glycation end product (AGE) treatment group (AGE group), and AGE-induced combined IL-8 antagonist SB225002 treatment group (AGE+SB group). The effect of AGE on IL-8 expression in hRCEC was observed by Western blot. The effect of SB225002 on hRCEC migration was observed by cell scratch assay. The effects of SB225002 on leukocyte adhesion and reactive oxygen species (ROS) on hRCEC were detected by flow cytometry. Student-t test was performed between the two groups. One-way analysis of variance was performed among the three groups. Results Compared with group N, the expression level of IL-8 in cells of AGE group was significantly increased, with statistical significance (t=25.661, P＜0.001). Compared with N group and AGE+SB group, cell mobility in AGE group was significantly increased (F=29.776), leukocyte adhesion number was significantly increased (F=38.159, 38.556), ROS expression level was significantly increased (F=22.336), and the differences were statistically significant (P＜0.05). Conclusion IL-8 antagonist SB225002 may down-regulate hRCEC adhesion and migration by inhibiting ROS expression.

Citation： Wang Linni, Cao Jingjing, Xing Dongjun, Yu Rongguo, Hu Liying, Yang Yang, Li Chang, Li Zhiqing, Li Hui, Hong Yaru, Dong Lijie. Interleukin-8 antagonist down regulates the adhesion and migration of retinal vascular endothelial cells by inhibiting the production of reactive oxygen species. Chinese Journal of Ocular Fundus Diseases, 2023, 39(11): 913-917. doi: 10.3760/cma.j.cn511434-20220315-00148 Copy

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1. Amadio M, Govoni S, Pascale A. Targeting VEGF in eye neovascularization: what's new?: A comprehensive review on current therapies and oligonucleotide-based interventions under development[J]. Pharmacol Res, 2016, 103: 253-269. DOI: 10.1016/j.phrs.2015.11.027.
2. Hernández-Zimbrón LF, Zamora-Alvarado R, Ochoa-De la Paz L, et al. Age-related macular degeneration: new paradigms for treatment and management of AMD[J/OL]. Oxid Med Cell Longev, 2018, 2018: 8374647[2018-02-01]. https://pubmed.ncbi.nlm.nih.gov/29484106/. DOI: 10.1155/2018/8374647.
3. Niu R, Nie ZT, Liu L, et al. Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathy[J]. Aging (Albany NY), 2021, 13(6): 8643-8664. DOI: 10.18632/aging.202678.
4. 黎仙, 李景云, 张媛, 等. IL-8在气道慢性炎症中的作用及研究进展[J]. 临床耳鼻咽喉头颈外科杂志, 2021, 35(12): 1144-1148. DOI: 10.13201/j.issn.2096-7993.2021.12.020.Li X, Li JY, Zhang Y, et al. The role of IL-8 in the chronic airway inflammation and its research progress[J]. Journal of Clinical Otorhinolaryngology Head and Neck Surgery, 2021, 35(12): 1144-1148. DOI: 10.13201/j.issn.2096-7993.2021.12.020.
5. Ghasemi H, Ghazanfari T, Yaraee R, et al. Roles of IL-8 in ocular inflammations: a review[J]. Ocul Immunol Inflamm, 2011, 19(6): 401-412. DOI: 10.3109/09273948.2011.618902.
6. Huang Q, Duan L, Qian X, et al. IL-17 promotes angiogenic factors IL-6, IL-8, and VEGF production via STAT1 in lung adenocarcinoma[J/OL]. Sci Rep, 2016, 6: 36551[2016-11-07]. https://pubmed.ncbi.nlm.nih.gov/27819281/. DOI: 10.1038/srep36551.
7. Lazzeri S, Orlandi P, Piaggi P, et al. IL-8 and VEGFR-2 polymorphisms modulate long-term functional response to intravitreal ranibizumab in exudative age-related macular degeneration[J]. Pharmacogenomics, 2016, 17(1): 35-39. DOI: 10.2217/pgs.15.153.
8. Shao Y, Chen J, Freeman W, et al. Canonical wnt signaling promotes neovascularization through determination of endothelial progenitor cell fate via metabolic profile eegulation[J]. Stem Cells, 2019, 37(10): 1331-1343. DOI: 10.1002/stem.3049.
9. 徐嫚鸿, 漆晨, 刘勋, 等. 聚嘧啶束结合蛋白相关剪接子高表达对糖基化终末产物诱导下人视网膜微血管内皮细胞氧化损伤的作用[J]. 中华眼底病杂志, 2020, 36(8): 633-640. DOI: 10.3760/cma.j.cn511434-20181012-00344.Xu MH, Qi C, Liu X, et al. The effects of highly expression of polypyramidine tract binding protein-associated splicing factor on advanced glycation end-products-induced human retinal microvascular endothelial cells[J]. Chin J Ocul Fundus Dis, 2020, 36(8): 633-640. DOI: 10.3760/cma.j.cn511434-20181012-00344.
10. 徐嫚鸿, 王林妮, 林婷婷, 等. 多聚嘧啶序列结合蛋白相关剪接因子对缺氧诱导人视网膜微血管内皮细胞功能的影响[J]. 中华眼底病杂志, 2020, 36(2): 135-142. DOI: 10.3760/cma.j.issn.1005-1015.2020.02.010.Xu MH, Wang LN, Lin TT, et al. Effects of pyrimidine bundle-binding protein-associated splicing factors on the function of hypoxiainduced human retinal microvascular endothelial cells[J]. Chin J Ocul Fundus Dis, 2020, 36(2): 135-142. DOI: 10.3760/cma.j.issn.1005-1015.2020.02.010.
11. Kobayashi Y. The role of chemokines in neutrophil biology[J]. Front Biosci, 2008, 13: 2400-2407. DOI: 10.2741/2853.
12. 张哲, 刘巨平, 东莉洁, 等. 高糖状态下视网膜血管内皮细胞基因表达谱的RNA-Seq分析[J]. 中华眼底病杂志, 2018, 34(4): 377-381. DOI: 10.3760/cma.j.issn.1005-1015.2018.04.014.Zhang Z, Liu JP, Dong LJ, et al. RNA-Seq analysis of gene expression profiling in retinal vascular endothelial cells under high glucose condition[J]. Chin J Ocul Fundus Dis, 2018, 34(4): 377-381. DOI: 10.3760/cma.j.issn.1005-1015.2018.04.014.
13. Kreuger J, Phillipson M. Targeting vascular and leukocyte communication in angiogenesis, inflammation and fibrosis[J]. Nat Rev Drug Discov, 2016, 15(2): 125-142. DOI: 10.1038/nrd.2015.2.
14. Pan SL, Tao KY, Guh JH, et al. The p38 mitogen-activated protein kinase pathway plays a critical role in PAR2-induced endothelial IL-8 production and leukocyte adhesion[J]. Shock, 2008, 30(5): 496-502. DOI: 10.1097/SHK.0b013e3181673233.
15. Do JY, Kim J, Kim MJ, et al. Fursultiamine alleviates choroidal neovascularization by suppressing inflammation and metabolic reprogramming[J]. Invest Ophthalmol Vis Sci, 2020, 61(12): 24. DOI: 10.1167/iovs.61.12.24.
16. 黄亮瑜, 柯屹峰, 林婷婷, 等. 慢病毒介导聚嘧啶束结合蛋白相关剪接因子对氧诱导视网膜病变小鼠视网膜新生血管的抑制作用[J]. 中华眼底病杂志, 2020, 36(1): 53-59. DOI: 10.3760/cma.j.issn.1005-1015.2020.01.012.Huang LY, Ke YF, Lin TT, et al. Lentivirus-mediated polypyrimidine bundle binding protein-associated splicing factor inhibits retinal neovascularization in mice of oxygen-induced retinopathy[J]. Chin J Ocul Fundus Dis, 2020, 36(1): 53-59. DOI: 10.3760/cma.j.issn.1005-1015.2020.01.012.
17. Jain SK, Micinski D, Parsanathan R. l-Cysteine stimulates the effect of Vitamin D on inhibition of oxidative stress, IL-8, and MCP-1 secretion in high glucose treated monocytes[J]. J Am Coll Nutr, 2021, 40(4): 327-332. DOI: 10.1080/07315724.2020.1850371.
18. Wang Y, Li H, Cao J, et al. BMP4 aggravates mitochondrial dysfunction of HRMECs[J/OL]. Heliyon, 2023, 9(3): e13824[2023-02-18]. https://pubmed.ncbi.nlm.nih.gov/36895361/. DOI: 10.1016/j.heliyon.2023.e13824.
19. Dong L, Li W, Lin T, et al. PSF functions as a repressor of hypoxia-induced angiogenesis by promoting mitochondrial function[J]. Cell Commun Signal, 2021, 19(1): 14. DOI: 10.1186/s12964-020-00684-w.
20. Feng W, Xue T, Huang S, et al. HIF-1α promotes the migration and invasion of hepatocellular carcinoma cells via the IL-8-NF-κB axis[J]. Cell Mol Biol Lett, 2018, 23: 26. DOI: 10.1186/s11658-018-0077-1.

Chinese Journal of Ocular Fundus Diseases

Interleukin-8 antagonist down regulates the adhesion and migration of retinal vascular endothelial cells by inhibiting the production of reactive oxygen species

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