Comparative study of the influence of silicone oil and heavy silicone on ultrastructure of rabbits' retina_Chinese Journal of Ocular Fundus Diseases

Authors：

DuanWenhua ,  WuMin

Department of Ophthalmology, The Second People's Hospital of Yunnan Province, Kunming 650021, China;

Corresponding author：

WuMin, Email: ynwumin@126.com

Keywords：

Silicone oils/adverse effects; Retina/ultrastructure; Retina/physiopathology; Animal experimentation

DOI：

10.3760/cma.j.issn.1005-1015.2015.03.016

Video：

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Objective To investigate the medium and long-term influence of silicon oil versus heavy silicone oil on rabbit retinas. Methods 28 health standard rabbits were randomly divided into A, B and C groups, with 12, 12 and 4 rabbits respectively. All rabbits received routine vitrectomy and tamponade with silicone oil (group A), or heavy silicone oil (group B) or balanced salt solution (group C). After 4, 8, 12 and 24 weeks, the retinal b-wave amplitude was measured by ERG, posterior retinal thickness was measured by optical coherence tomography (OCT). Retinal ultrastructure and tissue morphology were observed by transmission electron microscopy and optical microscopy. Results Compare to group C, the b-wave amplitude decreased at 4 weeks after surgery, and decreased at 8 weeks after surgery for group B, and decreased at 8 weeks after surgery, and decreased at 24 weeks after surgery for group A. The decreases were greater in group B than group A at 8, 12, 24 weeks after surgery, the difference was statistically significant (P < 0.05). The posterior retinal thickness of group A and B was thinner than group C at 24 weeks after surgery (P < 0.05). The decreases were greater in group B than group A, the difference was statistically significant (P < 0.05). Transmission electron microscopy and optical microscopy revealed severe pathological changes of retinal ultrastructure and morphology in group A and B rabbit eyes, at 12 weeks and 8 weeks after surgery respectively. The changes were more severe in group B and group A, including edema and necrosis in cone/rod cells, in disk membranes, mitochondria, cytoplasm, nucleus and other organelles. The morphological changes were also more severe in group B and group A, including degenerations of ganglion cell layer, inner nuclear layer changes. Those changes became more severe when the tamponade time extended. Conclusion The heavy silicone influence on visual function, ultrastructures, histomorphology of rabbit retinas is much worse than the silicon oil, and the effect is more significant with its time prolong.

Citation： DuanWenhua, WuMin. Comparative study of the influence of silicone oil and heavy silicone on ultrastructure of rabbits' retina. Chinese Journal of Ocular Fundus Diseases, 2015, 31(3): 279-284. doi: 10.3760/cma.j.issn.1005-1015.2015.03.016 Copy

1.	Sandner D, Engelmann K. First experiences with high-density silicone oil (Densiron) as an intraocular tamponade in complex retinal detachment[J]. Graefe's Arch Clin Exp Ophthalmol, 2006, 244(5):609-619.
2.	Joussen AM, Rizzo S, Kirchhof B, et al. Heavy silicone oil versus standard silicone oil in as vitreous tamponade in inferior PVR (HSO Study):interim analysis[J]. Acta Ophthalmologica, 2011, 89(6):483-489.
3.	Hussain RN, Banerjee S. Densiron 68 as an intraocular tamponade for complex inferior retinal detachments[J]. Clin Ophthalmol, 2011, 5(1):603-607.
4.	Li W, Zheng J, Zheng Q, et al. Clinical complications of Densiron 68 intraocular tamponade for complicated retinal detachment[J]. Eye, 2010, 24(1):21-28.
5.	Auriol S, Pagot-Mathis V, Mahieu L, et al. Efficacy and safety of heavy silicone oil Densiron 68 in the treatment of complicated retinal detachment with large inferior retinectomy[J]. Graefe's Arch Clin Exp Ophthalmol, 2008, 246(10):1383-1389.
6.	Kirchhof B. Strategies to influence PVR development[J]. Graefe's Arch Clin Exp Ophthalmol, 2004, 242(8):699-703.
7.	Wong D, van Meurs JC, Stappler T, et al. A pilot study on the use of a perfluorohexyloctan/silicone oil solution as a heavier than water internal tamponade agent[J].Br J Ophthalmol, 2005, 89(6):662-665.
8.	Dithmar S, Schuett F, Voelcker HE, et al. Delayed sequential occurrence of perfluorodecalin and silicone oil in the subretinal space following retinal detachment surgery in the presence of an optic disc pit[J]. Arch Ophthalmol, 2004, 122(3):409-411.
9.	Wickham L, Asaria RH, Alexander R, et al. Immunopathology of intraocular silicone oil:enucleated eyes[J]. Br J Ophthalmol, 2007, 91(2):253-257.
10.	Biswas J, Verma A, Davda MD, et a1.Intraocular tissue migration of silicone oil after silicone oil tamponade:a histopathological study of enucleated silicone oil-filled eyes[J]. Indian J Ophthalmol, 2008, 56(5):425-428.
11.	Knecht P, Groscurth P, Ziegler U, et a1.Is silicone oil optic neuropathy caused by high intraocular pressure alone? A semi-biological model[J]. Br J Ophthalmol, 2007, 91(10):1293-1295.
12.	Oliveira LB, Reis PA. Silicone oil tamponade in 23-gauge transconjunctival sutureless vitrectomy[J]. Retina, 2007, 27(8):1054-1058.
13.	Kampik A, Gandorfer A. Silicone oil removal strategies[J]. Semin Ophthalmol, 2000, 15(2):88-91.
14.	Theelen T, Tilanus MA, Klevering BJ.Intraocular inflammatio following endotamponade with high——density silicone oil[J].Graefe's Arch Clin Exp Ophthalmol, 2004, 242(7):617-620.
15.	Hotta K, Sugitani A. Refractive changes in silicone oil-filled pseudophakic eyes[J].Retina, 2005, 25(2):167-170.
16.	Eller AW, Friberg TR, Mah F. Migration of silicone oil into the brain:a complication of intraocular silicone oil for retinal tamponade[J]. Am J Ophthalmol, 2000, 129(5):685-688.
17.	Falkner CI, Binder S, Kruger A. Outcome after silicone oil removal[J]. Br J Ophthalmol, 2001, 85(11):1324-1327.
18.	Scott IU, Flynn HW, Murray TG, et al. Outcomes of complex retinal detachment repair using 1000-vs 5000-centistoke silicone oil[J]. Arch Ophthalmol, 2005, 123(4):473-478.
19.	Ichhpujani P, Jindal A, Jay Katz L. Silicone oil induced glaucoma:a review[J]. Graefe's Arch Clin Exp Ophthalmol, 2009, 247(12):1585-1593.
20.	La Heij EC, Hendrikse F, Kessels AG. Results and complications of temporary silicone oil tamponade in patients with complicated retinal detachments[J]. Retina, 2001, 21(2):107-114.
21.	Mackiewicz J, Mühling B, Hiebl W, et al. In vivo retinal tolerance of various heavy silicone oils[J]. Invest Ophthalmol Vis Sci, 2007, 48(4):1873-1883.
22.	Sandner D, Herbrig E, Engelmann K. High-density silicone oil (Densiron) as a primary intraocular tamponade:12-month follow up[J]. Graefe's Arch Clin Exp Ophthalmol, 2007, 245(8):1097-1105.
23.	Majid MA, Hussin HM, Biswas S, et al. Emulsification of Densiron-68 used in inferior retinal detachment surgery[J]. Eye (Lond), 2008, 22(1):152-157.
24.	Meng Q, Zhang S, Cheng H, et al. Long-term outcomes of Oxane Hd as intraocular tamponade in the treatment of complicated retinal detachment[J]. Graefe's Arch Clin Exp Ophthalmol, 2010, 248(8):1091-1096.

1. Sandner D, Engelmann K. First experiences with high-density silicone oil (Densiron) as an intraocular tamponade in complex retinal detachment[J]. Graefe's Arch Clin Exp Ophthalmol, 2006, 244(5):609-619.
2. Joussen AM, Rizzo S, Kirchhof B, et al. Heavy silicone oil versus standard silicone oil in as vitreous tamponade in inferior PVR (HSO Study):interim analysis[J]. Acta Ophthalmologica, 2011, 89(6):483-489.
3. Hussain RN, Banerjee S. Densiron 68 as an intraocular tamponade for complex inferior retinal detachments[J]. Clin Ophthalmol, 2011, 5(1):603-607.
4. Li W, Zheng J, Zheng Q, et al. Clinical complications of Densiron 68 intraocular tamponade for complicated retinal detachment[J]. Eye, 2010, 24(1):21-28.
5. Auriol S, Pagot-Mathis V, Mahieu L, et al. Efficacy and safety of heavy silicone oil Densiron 68 in the treatment of complicated retinal detachment with large inferior retinectomy[J]. Graefe's Arch Clin Exp Ophthalmol, 2008, 246(10):1383-1389.
6. Kirchhof B. Strategies to influence PVR development[J]. Graefe's Arch Clin Exp Ophthalmol, 2004, 242(8):699-703.
7. Wong D, van Meurs JC, Stappler T, et al. A pilot study on the use of a perfluorohexyloctan/silicone oil solution as a heavier than water internal tamponade agent[J].Br J Ophthalmol, 2005, 89(6):662-665.
8. Dithmar S, Schuett F, Voelcker HE, et al. Delayed sequential occurrence of perfluorodecalin and silicone oil in the subretinal space following retinal detachment surgery in the presence of an optic disc pit[J]. Arch Ophthalmol, 2004, 122(3):409-411.
9. Wickham L, Asaria RH, Alexander R, et al. Immunopathology of intraocular silicone oil:enucleated eyes[J]. Br J Ophthalmol, 2007, 91(2):253-257.
10. Biswas J, Verma A, Davda MD, et a1.Intraocular tissue migration of silicone oil after silicone oil tamponade:a histopathological study of enucleated silicone oil-filled eyes[J]. Indian J Ophthalmol, 2008, 56(5):425-428.
11. Knecht P, Groscurth P, Ziegler U, et a1.Is silicone oil optic neuropathy caused by high intraocular pressure alone? A semi-biological model[J]. Br J Ophthalmol, 2007, 91(10):1293-1295.
12. Oliveira LB, Reis PA. Silicone oil tamponade in 23-gauge transconjunctival sutureless vitrectomy[J]. Retina, 2007, 27(8):1054-1058.
13. Kampik A, Gandorfer A. Silicone oil removal strategies[J]. Semin Ophthalmol, 2000, 15(2):88-91.
14. Theelen T, Tilanus MA, Klevering BJ.Intraocular inflammatio following endotamponade with high——density silicone oil[J].Graefe's Arch Clin Exp Ophthalmol, 2004, 242(7):617-620.
15. Hotta K, Sugitani A. Refractive changes in silicone oil-filled pseudophakic eyes[J].Retina, 2005, 25(2):167-170.
16. Eller AW, Friberg TR, Mah F. Migration of silicone oil into the brain:a complication of intraocular silicone oil for retinal tamponade[J]. Am J Ophthalmol, 2000, 129(5):685-688.
17. Falkner CI, Binder S, Kruger A. Outcome after silicone oil removal[J]. Br J Ophthalmol, 2001, 85(11):1324-1327.
18. Scott IU, Flynn HW, Murray TG, et al. Outcomes of complex retinal detachment repair using 1000-vs 5000-centistoke silicone oil[J]. Arch Ophthalmol, 2005, 123(4):473-478.
19. Ichhpujani P, Jindal A, Jay Katz L. Silicone oil induced glaucoma:a review[J]. Graefe's Arch Clin Exp Ophthalmol, 2009, 247(12):1585-1593.
20. La Heij EC, Hendrikse F, Kessels AG. Results and complications of temporary silicone oil tamponade in patients with complicated retinal detachments[J]. Retina, 2001, 21(2):107-114.
21. Mackiewicz J, Mühling B, Hiebl W, et al. In vivo retinal tolerance of various heavy silicone oils[J]. Invest Ophthalmol Vis Sci, 2007, 48(4):1873-1883.
22. Sandner D, Herbrig E, Engelmann K. High-density silicone oil (Densiron) as a primary intraocular tamponade:12-month follow up[J]. Graefe's Arch Clin Exp Ophthalmol, 2007, 245(8):1097-1105.
23. Majid MA, Hussin HM, Biswas S, et al. Emulsification of Densiron-68 used in inferior retinal detachment surgery[J]. Eye (Lond), 2008, 22(1):152-157.
24. Meng Q, Zhang S, Cheng H, et al. Long-term outcomes of Oxane Hd as intraocular tamponade in the treatment of complicated retinal detachment[J]. Graefe's Arch Clin Exp Ophthalmol, 2010, 248(8):1091-1096.

Chinese Journal of Ocular Fundus Diseases

Comparative study of the influence of silicone oil and heavy silicone on ultrastructure of rabbits' retina

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