Protection of retinal ganglion cells from optic nerve injury by human umbilical cord blood stem cells transplantation_Chinese Journal of Ocular Fundus Diseases

Authors：

LiYunqin , XiaoLibo , KangJianshu , 张洁莹 , 周圆 ,  邹悦

Department of Ophtalmology, The Second People Hospital of Yunnan Province, Kunming 650021, China;

Corresponding author：

邹悦, Email: taoranzy@163.com

Keywords：

Optic nerve injuries; Optic nerve diseases/therapy; Cord blood stem cell transplantation

DOI：

10.3760/cma.j.issn.1005-1015.2015.03.017

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Objective To observe the protective effect of human umbilical cord blood stem cells (hUCBSC) transplantation on retinal ganglion cells (RGC) after optic nerve injury. Method 48 adult Sprague-Dawley rats were randomly divided into group A and B, therefore 24 rats in each group. Calibrated optic nerve crush injury model was induced in the left eyes, the right eyes served as a control. Medicine was injected at seventh day after optic nerve injury. PBS was injected into the eyes of Group A rats by peribulbar injection. The hUCBSCs were injected into the eyes of Group B rats by peribulbar injection. Seven days before sacrifice, 5% fluorogold was injected into superior colliculi bilaterally. At 7, 14, 21, 28 days after labeled, retinal flat mounts were observed under fluorescence microscope and optical microscope to investigate the morphological and RGC changes in density during retinal degeneration. Results The RGC number showed a tendency to decline gradually along with increases of the time in two groups, but the trend of decrease of Group B was evidently slower than that of Group A. The RGC number of the injury eye were less than the control eye in Group A and B (t=3.24, 3.15; P < 0.05). At 7, 14, 21, 28 days after labeled, the RGC number (t=4.78, 4.70, 3.98, 3.27; P < 0.05) and labeled RGC rate (t=4.39, 4.21, 4.36, 5.07; P < 0.05) in group B were more than those in group A. After optic nerve injury, there was karyopycnosis on ganglion cell layer of retina, thinning on each layer of retina, derangement of cell and decrease in RGC. There was different degree of the above change in different time after optic nerve injury. There were the swelling, the hemorrhage, derangement of spongiocyte and the denaturation like vacuole in the spot of optic nerve injury. Moreover, they were aggravating with increases of the time after optic nerve injury. There was no pathological changes in normal eyes. Conclusion The hUCBSC can increase the survival rate of the RGC and can rescue and(or) restore the injujed RGC after transplanted into body of optic nerve crush rat model by peribulbar injection.

Citation： LiYunqin, XiaoLibo, KangJianshu. Protection of retinal ganglion cells from optic nerve injury by human umbilical cord blood stem cells transplantation. Chinese Journal of Ocular Fundus Diseases, 2015, 31(3): 285-288. doi: 10.3760/cma.j.issn.1005-1015.2015.03.017 Copy

1.	Gluckman E, Broxmeyer HA, Auerbach AD, et al.Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical-cord blood from an HLA-identical sibling[J]. Nengl J Med, 1989, 321(17):1174-1178.
2.	Yamasaki TR, Blurton-Jones M, Morrissette DA, et al. Neural stem cells improve memory in an inducible mouse model of neuronal loss[J]. J Neurosci, 2007, 27(44):11925-11933.
3.	Zhao T, Li Y, Tang L, et al. Protective effect of human umbilical cord blood stem cell intravitreal transplantation against optic nerve injury in rats[J].Graefe's Arch Exp Ophthalmol, 2011, 249(7):1021-1028.
4.	Elsaeidi F, Bemben MA, Zhao XF, et al. Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq[J]. J Neurosci, 2014, 34(7):2632-2644.
5.	Gluckman E. Hematopoietic stem cell transplangtsusing umbilical cord blood[J]. Nengl J Med, 2001, 344(24):1860-1861.
6.	Garbuzova-Davis S, Willing AE, Zigova T, et al.Intravenous administration of human umbilical cord blood cells in a mouse model of amyotrophic lateral sclerosis:distribution, migration, and differentiation[J].J Hematother Stem Cell Res, 2003, 12(3):255-270.
7.	史剑波, 徐锦堂, 夏潮勇.视神经损伤对视网膜结构的形态学研究[J].眼外伤职业眼病杂志, 2004, 20(4):283-285.
8.	Wanger JE, Barker JN, de For TE, et al.Transplantion of unrelated onor umbilical cord blood in 102 patients with malignant and non malignant disease:influence of CD34 cell dose and HLA disparity on treatment related mortality and survival[J]. Blood, 2002, 100(5):1611-1618.
9.	Saporta S, Kim JJ, Willing AE, et a1.Human umbilical cord blood stem cells infusion in spinal cord injury:engraftment and beneficial influence on behavior[J].J Hematother Stem Cell Res, 2003, 12(3):271-278.
10.	李洪钧, 刘海英, 赵宗茂, 等.人脐血干细胞移植促进大鼠脊髓损伤神经恢复[J].中国医学科学院学报, 2004, 2(1):38-42.

1. Gluckman E, Broxmeyer HA, Auerbach AD, et al.Hematopoietic reconstitution in a patient with Fanconi's anemia by means of umbilical-cord blood from an HLA-identical sibling[J]. Nengl J Med, 1989, 321(17):1174-1178.
2. Yamasaki TR, Blurton-Jones M, Morrissette DA, et al. Neural stem cells improve memory in an inducible mouse model of neuronal loss[J]. J Neurosci, 2007, 27(44):11925-11933.
3. Zhao T, Li Y, Tang L, et al. Protective effect of human umbilical cord blood stem cell intravitreal transplantation against optic nerve injury in rats[J].Graefe's Arch Exp Ophthalmol, 2011, 249(7):1021-1028.
4. Elsaeidi F, Bemben MA, Zhao XF, et al. Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq[J]. J Neurosci, 2014, 34(7):2632-2644.
5. Gluckman E. Hematopoietic stem cell transplangtsusing umbilical cord blood[J]. Nengl J Med, 2001, 344(24):1860-1861.
6. Garbuzova-Davis S, Willing AE, Zigova T, et al.Intravenous administration of human umbilical cord blood cells in a mouse model of amyotrophic lateral sclerosis:distribution, migration, and differentiation[J].J Hematother Stem Cell Res, 2003, 12(3):255-270.
7. 史剑波, 徐锦堂, 夏潮勇.视神经损伤对视网膜结构的形态学研究[J].眼外伤职业眼病杂志, 2004, 20(4):283-285.
8. Wanger JE, Barker JN, de For TE, et al.Transplantion of unrelated onor umbilical cord blood in 102 patients with malignant and non malignant disease:influence of CD34 cell dose and HLA disparity on treatment related mortality and survival[J]. Blood, 2002, 100(5):1611-1618.
9. Saporta S, Kim JJ, Willing AE, et a1.Human umbilical cord blood stem cells infusion in spinal cord injury:engraftment and beneficial influence on behavior[J].J Hematother Stem Cell Res, 2003, 12(3):271-278.
10. 李洪钧, 刘海英, 赵宗茂, 等.人脐血干细胞移植促进大鼠脊髓损伤神经恢复[J].中国医学科学院学报, 2004, 2(1):38-42.

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Chinese Journal of Ocular Fundus Diseases

Protection of retinal ganglion cells from optic nerve injury by human umbilical cord blood stem cells transplantation

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