The relationship between the expression ratio of heat shock protein 70 to C-fos in the main organs outside the brain and the time of injury after brain concussion in rats_West China Medical Journal

Authors：

JIN Wei , XIA Yongjun , LI Xuerong , LIN Wei ,  LIU Min

West China School of Basic and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P. R. China;

Corresponding author：

LIU Min, Email: min8liu@hotmail.com

Keywords：

C-fos; Heat shock protein 70; Brain concussion; Injury time determination; Ratio of positive cell; Rats

DOI：

10.7507/1002-0179.201608126

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Objective To study the relationship between the expression ratio of heat shock protein (HSP) 70 to C-fos in organs outside the brain after brain concussion and the time of injury in rats, in order to provide a new visual angle for determining injury time of brain concussion. Methods The model of brain concussion was established through free falling method. Then the rats were executed at 30 minutes, 1 hour, and 3, 6, 12, 24, 48, 96, 168, 240, 336 hours after injury. Immunohistochemistry staining of C-fos and HSP70 were used in the materials from the main organs including heart, liver, spleen, lung and kidney. All related experiment results were studied by using a microscope with image analytical system and homologous statistics. Results From 30 minutes to 6 hours after injury, the proportion of HSP70 immuno-positive cells increased slowly, while the proportion of C-fos immuno-positive cells increased rapidly, and the ratio of HSP70/C-fos positive cells was on the decline. From 6 to 12 hours after injury, the proportion of HSP70 immuno-positive cells rose continuously, while the proportion of C-fos immuno-positive cells started to decrease, and the HSP70/C-fos ratio showed a rising tendency. From 12 to 336 hours after injury, the proportion of HSP70 immuno-positive cells decreased slowly, while the proportion of C-fos immuno-positive cells decreased rapidly, and the HSP70/C-fos ratio was still on the rise. Conclusions The proportion of positive cells and ratio of the two markers in the main organs including heart, liver, spleen, lung and kidney are similar to those in the brain of rats after brain concussion. Observing the proportion of positive cells of the two markers together with their ratio in the main organs outside the brain may provide a reference for the determination of injury time after brain concussion.

Citation： JIN Wei, XIA Yongjun, LI Xuerong, LIN Wei, LIU Min. The relationship between the expression ratio of heat shock protein 70 to C-fos in the main organs outside the brain and the time of injury after brain concussion in rats. West China Medical Journal, 2017, 32(10): 1539-1543. doi: 10.7507/1002-0179.201608126 Copy

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1. 严治, 孙小丽, 胡玉莲, 等. 大鼠脑挫伤后脑与其他器官组织中 TNF-α 表达的比较. 法医学杂志, 2012(4): 261-264.
2. 汪枫, 李永宏, 胡永良. 实验性大鼠脑震荡后 C-FOS 蛋白表达的研究. 法医学杂志, 2003, 19(1): 8-9.
3. 李菁. 实验性大鼠脑震荡后 C-FOS 蛋白表达. 中国现代医学杂志, 2003, 13(21): 27-29.
4. 董贺文, 代号, 夏勇军, 等. 大鼠脑震荡伤后诱导型一氧化氮合酶与胶质纤维酸性蛋白表达比值随损伤时间变化的关系研究. 华西医学, 2016, 31(4): 718-723.
5. 陈锐, 严治, 胡玲, 等. 大鼠脑震荡模型建立法的改良和评价. 广东医学院学报, 2010, 28(4): 362-363.
6. Landschuiz WH, Johnson PF, McKnight SL. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science, 1988, 240(4860): 1759-1764.
7. Sato S, Abe K, Kanagoe J, et al. Isolation of complementary DNAs for heat shock protein (HSP) 70 and heat shock cognate protein (HSC) 70 genes and the expressions in post-ischaemic gerbil brain. Neurol Res, 1992, 14(5): 375-380.
8. Raghupathi R, Muir JK, Fulp CT, et al. Acute activation of mitogen-activated protein kinases following traumatic brain injury in the rat: implications for post traumatic cell death. Exp Neurol, 2003, 183(2): 438-448.
9. Love S. Apoptosis and brain ischaemia. Prog Neuropsychopharmacol Biol Psychiatry, 2003, 27(2): 267-282.
10. 赵连旭, 徐小虎, 潘速跃, 等. 脑损伤后热休克蛋白 70 及热休克因子 Ⅰ 基因转录的实验研究. 解剖学研究, 2002, 24(1): 9-11.
11. 陈锐, 余彬华, 胡玲, 等. 大鼠脑震荡伤后脑组织 HSP70、bFGF 及 TGF-β1 表达变化. 法医学杂志, 2009, 25(4): 249-253.

West China Medical Journal

The relationship between the expression ratio of heat shock protein 70 to C-fos in the main organs outside the brain and the time of injury after brain concussion in rats

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