Magnetic nanoparticle method for detecting circulating tumor cells of lung cancer: A pilot study_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

XU Yuan ¹ , LIANG Naixin ¹ , LIU Hongsheng ¹ , LI Li ¹ , HUANG Cheng ¹ , QIN Yingzhi ¹ , BING Zhongxing ¹ , PENG Jiaqian ² , LI Wenzhe ² , YANG Yanlian ² , HU Zhiyuan ² ,  LI Shanqing ¹

1. Department of Thoracic surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, 100730, P.R.China;
2. National Center for Nanoscience and Technology, Beijing, 100190, P.R.China;

Corresponding author：

LI Shanqing, Email: lsq6768@sohu.com

Keywords：

Circulating tumor cells; magnetic nanoparticle method; lung cancer

DOI：

10.7507/1007-4848.201708052

Video：

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Objective To explore the diagnostic value of circulating tumor cells (CTC) measured by magnetic nanoparticle method in lung cancer. Methods (1) We measured binding capability of A549 or NCI-H1965 cell lines with recognition peptide and capture efficiency by adding tumor cells into the whole blood of healthy human. (2) We measured CTC of 34 patients suspected with lung cancer, and the counting results of CTC were compared with the following pathological results. Results (1) The binding capability was 80.0%±6.0% for A549 and 70.1%±4.8% for H1957, while the capture efficiency was 57.3%±7.0% for A549 and 37.3%±6.1% for H1975. (2) CTCs were identified in 71.9% of patients with lung cancer. The specificity was 83.3%, and area under receiver operating characteristic (ROC) curve was 0.792 (P=0.003). Conclusion CTC measured by magnetic nanoparticle method has promising application in the diagnosis of lung cancer.

Citation： XU Yuan, LIANG Naixin, LIU Hongsheng, LI Li, HUANG Cheng, QIN Yingzhi, BING Zhongxing, PENG Jiaqian, LI Wenzhe, YANG Yanlian, HU Zhiyuan, LI Shanqing. Magnetic nanoparticle method for detecting circulating tumor cells of lung cancer: A pilot study. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2018, 25(8): 691-695. doi: 10.7507/1007-4848.201708052 Copy

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9.	Pak MG, Shin DH, Lee CH, et al. Significance of EpCAM and TROP2 expression in non-small cell lung cancer. World J Surg Oncol, 2012, 10: 53.
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12.	Fiorelli A, Accardo M, Carelli E, et al. Circulating tumor cells in diagnosing lung cancer: clinical and morphologic analysis. Ann Thorac Surg, 2015, 99(6): 1899-1905.
13.	Ma XL, Xiao ZL, Liu L, et al. Meta-analysis of circulating tumor cells as a prognostic marker in lung cancer. Asian Pac J Cancer Prev, 2012, 13(4): 1137-1144.
14.	Wang J, Wang K, Xu J, et al. Prognostic significance of circulating tumor cells in non-small-cell lung cancer patients: a meta-analysis. PLoS One, 2013, 8(11): e78070.

1. Ashworth TR. A case of cancer in which cells similar to those in the tumours were seen in the blood after death. Aust Med J, 1869, 14: 146-147.
2. Hamilton G, Rath B. Detection of circulating tumor cells in non-small cell lung cancer. J Thorac Dis, 2016, 8(6): 1024-1028.
3. Bai LL, Du YM, Peng JX, et al. Peptide-based isolation of circulating tumor cells by magnetic nanoparticles. J Mat Chem B, 2014, 2(26): 4080-4089.
4. Tanaka F, Yoneda K, Kondo N, et al. Circulating tumor cell as a diagnostic marker in primary lung cancer. Clin Cancer Res, 2009, 15(22): 6980-6986.
5. Isobe K, Hata Y, Kobayashi K, et al. Clinical significance of circulating tumor cells and free DNA in non-small cell lung cancer. Anticancer Res, 2012, 32(8): 3339-3344.
6. Hofman V, Ilie MI, Long E, et al. Detection of circulating tumor cells as a prognostic factor in patients undergoing radical surgery for non-small-cell lung carcinoma: comparison of the efficacy of the CellSearch Assay^TM and the isolation by size of epithelial tumor cell method. Int J Cancer, 2011, 129(7): 1651-1660.
7. Lecharpentier A, Vielh P, Perez-Moreno P, et al. Detection of circulating tumour cells with a hybrid (epithelial/mesenchymal) phenotype in patients with metastatic non-small cell lung cancer. Br J Cancer, 2011, 105(9): 1338-1341.
8. De Wit S, Van Dalum G, Lenferink ATM, et al. Abstract 377: EpCAM+ and EpCAM- circulating tumor cells in metastatic lung cancer. Canc Res, 2015, 75(15 Suppl): 377.
9. Pak MG, Shin DH, Lee CH, et al. Significance of EpCAM and TROP2 expression in non-small cell lung cancer. World J Surg Oncol, 2012, 10: 53.
10. Calabuig-Fariñas S, Jantus-Lewintre E, Herreros-Pomares A, et al. Circulating tumor cells versus circulating tumor DNA in lung cancer-which one will win? Transl Lung Cancer Res, 2016, 5(5): 466-482.
11. Lou J, Zhou C, Wu J. Abstract 2247: A multicenter clinical trial of lung cancer circulating tumor cell assay with the largest sample size (1210 cases) in China. Canc Res, 2016, 76(14 Suppl): 2247.
12. Fiorelli A, Accardo M, Carelli E, et al. Circulating tumor cells in diagnosing lung cancer: clinical and morphologic analysis. Ann Thorac Surg, 2015, 99(6): 1899-1905.
13. Ma XL, Xiao ZL, Liu L, et al. Meta-analysis of circulating tumor cells as a prognostic marker in lung cancer. Asian Pac J Cancer Prev, 2012, 13(4): 1137-1144.
14. Wang J, Wang K, Xu J, et al. Prognostic significance of circulating tumor cells in non-small-cell lung cancer patients: a meta-analysis. PLoS One, 2013, 8(11): e78070.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Magnetic nanoparticle method for detecting circulating tumor cells of lung cancer: A pilot study

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