Role of Vasoactive Intestinal Peptide in Relaxation of the Lower Esophageal Sphincter in Human_Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Authors：

LI Yunfeng , LIU Junfeng.

Third Ward of Department of Thoracic Surgery， Fourth Affiliated Hospital， Hebei Medical University，　Shijiazhuang 050011， P. R. China;

Corresponding author：

Keywords：

Lower esophageal sphincter; 　Sling fiber; 　Clasp fiber; 　Nonadrenergic and noncholinergic; 　Vasoactive intestinal peptide; 　Electric field stimulation

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

Abstract：　Objective　To investigate the influence of vasoactive intestinal peptide （VIP) on the sling fibers and the clasp fibers of the lower esophageal sphincter （LES） and the difference， and explore whether VIP belongs to a nonadrenergic　and noncholinergic （NANC） neurotransmitter.　Methods　Thirty LES specimens were obtained from 30 patients with high-position carcinoma of the middle thoracic esophagus who underwent esophagectomy from March to August 2010 in Fourth Affiliated Hospital of Hebei Medical University. There were 14 male patients and 16 female patients with their average　age of 58.0±6.1 years. The clasp fibers and sling fibers were isolated and suspended in perfusion. Exogenous VIP was added to the two kinds of strips to draw a concentration-effect curve. Electric field stimulation （EFS） or exogenous VIP was applied to clasp fibers and sling fibers， and the influence of VIP （10-28） on LES was compared.　Results　Exogenous
VIP in different concentration caused concentration-dependent relaxation of the sling fibers and clasp fibers of LES in vitro.　There was statistical difference in relaxation between the sling fibers and clasp fibers under same VIP concentration （P＜0.05），　and the relaxation of sling fibers was more significant than that of clasp fibers. VIP （10-28） transiently inhibited the relaxation
of the sling fibers and clasp fibers caused by exogenous VIP. VIP （10-28） also transiently inhibited the relaxation of the sling fibers and clasp fibers after the activation of EFS.　Conclusion　The relaxation of sling fibers and clasp fibers induced by　EFS is related to VIP. VIP is a kind of NANC neurotransmitter in human LES.

Citation： LI Yunfeng,LIU Junfeng.. Role of Vasoactive Intestinal Peptide in Relaxation of the Lower Esophageal Sphincter in Human. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2012, 19(6): 654-658. doi: Copy

1.	Liebermann-Meffert D， Allgöwer M， Schmid P， et al. Muscular equivalent of the lower esophageal sphincter. Gastroenterology， 1979， 76 （1）：31-38.
2.	Stein HJ， Korn O， Liebermann-Meffert D. Manometric vector　volume analysis to assess lower esophageal sphincter function. Ann Chir Gynaecol， 1995， 84 （2）：151-158.
3.	Welch RW， Drake ST. Normal lower esophageal sphincter pressure：　a comparison of rapid vs. slow pull-through techniques.　Gastroenterology， 1980， 78 （6）：1446-1451.
4.	Muinuddin A， Xue S， Diamant NE. Regional differences in the　response of feline esophageal smooth muscle to stretch and cholinergic　stimulation. Am J Physiol Gastrointest Liver Physiol， 2001， 281 （6）：G1460-G1467.
5.	Tian ZQ， Liu JF， Wang GY， et al. Responses of human clasp and sling fibers to neuromimetics. J Gastroenterol Hepatol， 2004， 19 （4）：440-447.
6.	Christensen J， Lund GF. Esophageal responses to distension and electrical stimulation. J Clin Invest， 1969， 48 （2）：408-419.
7.	Murray J， Du C， Ledlow A， et al. Nitric oxide：mediator of　nonadrenergic noncholinergic responses of opossum esophageal　muscle. Am J Physiol， 1991， 261 （3 Pt 1）：G401-G406.
8.	Harmar AJ， Arimura A， Gozes I， et al. International union of pharmacology. XⅧ. Nomenclature of receptors for vasoactive intestinal　peptide and pituitary adenylate cyclase-activating polypeptide.　Pharmacol Rev， 1998， 50 （2）：265-270.
9.	Aggestrup S， Uddman R， Steen LindkÃr Jensen， et al. Regulatory peptides in the lower esophageal sphincter of man. Regul Pept， 1985， 10 （2â）：167-178.
10.	Ny L， Alm P， Larsson B， et al. Nitric oxide pathway in cat esophagus：　localization of nitric oxide synthase and functional effects. Am J Physiol， 1995， 268 （1 Pt 1）：G59-G70.
11.	Rattan S， Grady M， Goyal RK. Vasoactive intestinal peptide causes peristaltic contractions in the esophageal body. Life Sci， 1982， 30 （18）：1557-1563.
12.	Guelrud M， Rossiter A， Souney PF， et al. The effect of vasoactive intestinal polypeptide on the lower esophageal sphincter in achalasia. Gastroenterology， 1992， 103 （2）：377-382.
13.	Jury J， Ahmedzadeh N， Daniel EE. A mediator derived from arginine　mediates inhibitory junction potentials and relaxations in lower　esophageal sphincter：an Independent role for vasoactive intestinal peptide. Can J Physiol Pharmacol， 1992， 70 （8）：1182-1189.
14.	Uc A， Oh ST， Murray JA， et al. Biphasic relaxation of the opossum lower esophageal sphincter：roles of NO. VIP， and CGRP. Am J Physiol， 1999， 277 （3 Pt 1）：G548-G554.
15.	Farré R， Aulí M， Lecea B， et al. Pharmacologic characterization of intrinsic mechanisms controlling tone and relaxation of porcine lower esophageal sphincter. J Pharmacol Exp Ther， 2006， 316 （3）：1238-1248.
16.	Jun CH， Lee TS， Sohn UD. NO/cyclic GMP pathway mediates the relaxation of feline lower oesophageal sphincter. Auton Autacoid Pharmacol， 2003， 23 （3）：159-166.
17.	Desai KM， Warner TD， Bishop AE， et al. Nitric oxide， and not vasoactive intestinal peptide， as the main neurotransmitter of vagally induced relaxation of the Guinea pig stomach. Br J Pharmacol， 1994， 113 （4）：1197-1202.
18.	谢勇，黄缘，王崇文. 下食管括约肌压力与局部组织内一氧化氮和血管活性肠肽含量的关系. 中华消化内镜杂志， 1999， 16 （6）：334.
19.	Jin JG， Murthy KS， Grider JR， et al. Activation of distinct cAMP- and cGMP-dependent pathways by relaxant agents in isolated gastric muscle cells. Am J Physiol， 1993， 264 （3 Pt 1）：G470-G477.
20.	Grider JR. Interplay of VIP and nitric oxide in regulation of the　descending relaxation phase of peristalsis. Am J Physiol， 1993， 264 （2 Pt 1）：G334-G340.
21.	温士旺，刘俊峰，高立平，等. 一氧化氮介导人食管下括约肌舒张的作用机制. 中华医学杂志， 2006， 86 （1）：31-34.
22.	Turner JT， Jones SB， Bylund DB. A fragment of vasoactive intestinal peptide， VIP （10-28）， is an antagonist of VIP in the colon carcinoma cell line， HT29. Peptides， 1986， 7 （5）：849-854.
23.	Chakder S， Rattan S. The entire vasoactive intestinal polypeptide molecule is required for the activation of the vasoactive intestinal polypeptide receptor：functional and binding studies on opossum internal anal sphincter smooth muscle. J Pharmacol Exp Ther， 1993， 266 （1）：392-399.

1. 　Liebermann-Meffert D， Allgöwer M， Schmid P， et al. Muscular equivalent of the lower esophageal sphincter. Gastroenterology， 1979， 76 （1）：31-38.
2. 　Stein HJ， Korn O， Liebermann-Meffert D. Manometric vector　volume analysis to assess lower esophageal sphincter function. Ann Chir Gynaecol， 1995， 84 （2）：151-158.
3. 　Welch RW， Drake ST. Normal lower esophageal sphincter pressure：　a comparison of rapid vs. slow pull-through techniques.　Gastroenterology， 1980， 78 （6）：1446-1451.
4. 　Muinuddin A， Xue S， Diamant NE. Regional differences in the　response of feline esophageal smooth muscle to stretch and cholinergic　stimulation. Am J Physiol Gastrointest Liver Physiol， 2001， 281 （6）：G1460-G1467.
5. 　Tian ZQ， Liu JF， Wang GY， et al. Responses of human clasp and sling fibers to neuromimetics. J Gastroenterol Hepatol， 2004， 19 （4）：440-447.
6. 　Christensen J， Lund GF. Esophageal responses to distension and electrical stimulation. J Clin Invest， 1969， 48 （2）：408-419.
7. 　Murray J， Du C， Ledlow A， et al. Nitric oxide：mediator of　nonadrenergic noncholinergic responses of opossum esophageal　muscle. Am J Physiol， 1991， 261 （3 Pt 1）：G401-G406.
8. 　Harmar AJ， Arimura A， Gozes I， et al. International union of pharmacology. XⅧ. Nomenclature of receptors for vasoactive intestinal　peptide and pituitary adenylate cyclase-activating polypeptide.　Pharmacol Rev， 1998， 50 （2）：265-270.
9. 　Aggestrup S， Uddman R， Steen LindkÃr Jensen， et al. Regulatory peptides in the lower esophageal sphincter of man. Regul Pept， 1985， 10 （2â）：167-178.
10. 　Ny L， Alm P， Larsson B， et al. Nitric oxide pathway in cat esophagus：　localization of nitric oxide synthase and functional effects. Am J Physiol， 1995， 268 （1 Pt 1）：G59-G70.
11. 　Rattan S， Grady M， Goyal RK. Vasoactive intestinal peptide causes peristaltic contractions in the esophageal body. Life Sci， 1982， 30 （18）：1557-1563.
12. 　Guelrud M， Rossiter A， Souney PF， et al. The effect of vasoactive intestinal polypeptide on the lower esophageal sphincter in achalasia. Gastroenterology， 1992， 103 （2）：377-382.
13. 　Jury J， Ahmedzadeh N， Daniel EE. A mediator derived from arginine　mediates inhibitory junction potentials and relaxations in lower　esophageal sphincter：an Independent role for vasoactive intestinal peptide. Can J Physiol Pharmacol， 1992， 70 （8）：1182-1189.
14. 　Uc A， Oh ST， Murray JA， et al. Biphasic relaxation of the opossum lower esophageal sphincter：roles of NO. VIP， and CGRP. Am J Physiol， 1999， 277 （3 Pt 1）：G548-G554.
15. 　Farré R， Aulí M， Lecea B， et al. Pharmacologic characterization of intrinsic mechanisms controlling tone and relaxation of porcine lower esophageal sphincter. J Pharmacol Exp Ther， 2006， 316 （3）：1238-1248.
16. 　Jun CH， Lee TS， Sohn UD. NO/cyclic GMP pathway mediates the relaxation of feline lower oesophageal sphincter. Auton Autacoid Pharmacol， 2003， 23 （3）：159-166.
17. 　Desai KM， Warner TD， Bishop AE， et al. Nitric oxide， and not vasoactive intestinal peptide， as the main neurotransmitter of vagally induced relaxation of the Guinea pig stomach. Br J Pharmacol， 1994， 113 （4）：1197-1202.
18. 　谢勇，黄缘，王崇文. 下食管括约肌压力与局部组织内一氧化氮和血管活性肠肽含量的关系. 中华消化内镜杂志， 1999， 16 （6）：334.
19. 　Jin JG， Murthy KS， Grider JR， et al. Activation of distinct cAMP- and cGMP-dependent pathways by relaxant agents in isolated gastric muscle cells. Am J Physiol， 1993， 264 （3 Pt 1）：G470-G477.
20. 　Grider JR. Interplay of VIP and nitric oxide in regulation of the　descending relaxation phase of peristalsis. Am J Physiol， 1993， 264 （2 Pt 1）：G334-G340.
21. 　温士旺，刘俊峰，高立平，等. 一氧化氮介导人食管下括约肌舒张的作用机制. 中华医学杂志， 2006， 86 （1）：31-34.
22. 　Turner JT， Jones SB， Bylund DB. A fragment of vasoactive intestinal peptide， VIP （10-28）， is an antagonist of VIP in the colon carcinoma cell line， HT29. Peptides， 1986， 7 （5）：849-854.
23. 　Chakder S， Rattan S. The entire vasoactive intestinal polypeptide molecule is required for the activation of the vasoactive intestinal polypeptide receptor：functional and binding studies on opossum internal anal sphincter smooth muscle. J Pharmacol Exp Ther， 1993， 266 （1）：392-399.

Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

Role of Vasoactive Intestinal Peptide in Relaxation of the Lower Esophageal Sphincter in Human

Abstract Full text Figures/Tables Video References Cited by

Format

Content