HE Gengxu 1 , YAO Tong 2 , ZHANG Hao 3
  • (1.Department of Thoracic and Cardiovascular Surgery, 251 Hospital of People’s Liberation Army, Zhangjiakou 075000, Hebei, P.R. China;;
  • 2.Department of Cardiac, the First Affiliated Hospital, Hebei North College, Zhangjiakou 075000, Hebei, P.R. China;;
  • 3. Research Center for Cardiovascular Regeneration Medicine of the Ministry of Health, Fu Wai Cardiovascular Hospital，Chinese Academy of Medical Sciences &;
  • Peking Union Medical College， Beijing 100037， P．R．China);
Bone marrow mesenchymal stem cell; Cell transplantation; Coronary artery; Magnetic resonance imaging; Superparamagnetic iron oxide 
Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables References Cited by

Objective To investigate the feasibility of imaging of bone marrow mesenchymal stem cells (BMMSCs) labeled with superparamagnetic iron oxide(SPIO) transplanted into coronary artery in vivo using magnetic resonance imaging (MRI), and the redistribution of the cells into other organs. Methods BMMSCs were isolated, cultured from bone marrow of Chinese mini swine, and double labeled with SPIO and CMDiI(Cell TrackerTM C-7001). The labeled cells were injected into left anterior descending coronary artery through a catheter. The injected cells were detected by using MRI at 1 week,3weeks after transplantation. And different organs were harvested and evaluated the redistribution of transplanted cells through pathology. Results The SPIO labeled BMMSCs injected into coronary artery could be detected through MRI and confirmed by pathology and maintained more than 3 weeks. The SPIO labeled cells could be clearly imaged as signal void lesions in the related artery. The pathology showed that the injected cells could be distributed into the area of related artery, and the cells injected into coronary artery could be found in the lung, spleen, kidney, but scarcely in the liver, the structures of these organs remained normal. Conclusion The SPIO labeled BMMSCs injected into coronary artery can be detected by using MRI, the transplanted cells can be redistributed into the non-targeted organs.

Citation： HE Gengxu,YAO Tong,ZHANG Hao,HU Shengshou,SONG Peng,ZHANG Xiaoling. The Experimental Study of Imaging and Redistribution of Bone Marrow Mesenchymal Stem Cells Transplanted into Coronary Artery in Vivo. Chinese Journal of Clinical Thoracic and Cardiovascular Surgery, 2008, 15(3): 195-199. doi: Copy