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find Keyword "亚低温" 12 results
  • TREATMENT OF SPINAL CORD INJURY BY MILD HYPOTHERMIA COMBINED WITH BONE MARROW MESENCHYMAL STEM CELLS TRANSPLANTATION IN RATS

    Objective Bone marrow mesenchymal stem cells (BMSCs) play an important role in repairing nerve injury, meanwhile external temperature has significant effect on BMSCs transplantation, prol iferation, and differentiation. To investigate the effect of BMSCs transplantation and mild hypothermia on repair of rat spinal cord injury (SCI). Methods Forty-five female adult SD rats (weighing 200-250 g) were made the models of hemitransection SCI and divided randomly into 3 groups according to different treatments: group A (SCI group), group B (BMSCs transplantation group), and group C [BMSCs transplantation combined with mild hypothermia (33-35 ) group]. At 1, 2, 4, 6, and 8 weeks after injury, the fuction of hind l imb was evaluated with Basso Beattie and Bresnahan (BBB) score and incl ined plane test. At 4 weeks after injury, histopathology and BrdU immunohistochemistry staining were performed. At 8 weeks after injury, horseradishperoxidase (HRP) retrograde nerve trace and transmission electron microscope (TEM) testing were performed to observe the regeneration of axon. Results After 4 weeks, the function of hind l imb obviously recovered in groups B and C, there were significant differences in BBB score between groups B, C and group A (P lt; 0.05), between group B and group C (P lt; 0.05). There was no significant difference (P gt; 0.05) in tilt angle among 3 groups after 1 and 2 weeks, and there were significant differences (P lt; 0.05) among 3 groups after 4 weeks. HE staining showed that significant cavity could be seen in group A, l ittle in group B, and no cavity in group C. BrdU immunohistochemistry staining showed that the number of positive cells was 0, 90.54 ± 6.23, and 121.22 ± 7.54 in groups A, B, and C, respectively; showing significant differences (P lt; 0.01) among 3 groups. HRP retrograde neural tracing observation showed that the number of HRP positive nerve fibers was 10.35 ± 1.72, 43.25 ± 2.65, and 84.37 ± 4.59 in groups A, B, and C, respectively, showing significant differences (P lt; 0.01) among 3 groups. TEM observation showed that a great amount of unmyel inated nerve fibers and myel inated nerve fibers were found in central transverse plane in group C. Conclusion The BMSCs transplantation play an impontant role in promotion of recovering the function of hind l imb after SCI, and mild hypothermia has synergism effects.

    Release date:2016-08-31 05:48 Export PDF Favorites Scan
  • 显微血肿清除及去大骨瓣减压手术联合亚低温和腰大池引流治疗重症原发性脑出血

    目的 总结应用显微血肿清除及去大骨瓣减压手术联合亚低温和腰大池引流治疗重症原发性脑出血的临床效果。 方法 对2010年2月-2012年2月141例重症原发性脑出血患者,分别采用保守治疗(保守组32例)、常规开颅显微手术血肿清除治疗(对照组55例)及去大骨瓣显微手术联合亚低温及腰大池引流治疗(治疗组54例)三种方法进行治疗,并对3个月后的病死率及日常生活活动能力(ADL)进行比较分析。 结果 结果保守组32患者中死亡17例(病死率53.1%),对照组55例患者中死亡22例(病死率40.0%),治疗组54例患者中死亡19例(病死率35.2%),3组间患者病死率差异有统计学意义(P<0.05),治疗组的病死率明显低于保守组和对照组,差异均有统计学意义(P<0.05)。治疗组ADL分级1~3级患者明显多于保守组和对照组(P<0.01)。 结论 显微血肿清除及去大骨瓣减压手术联合亚低温和腰大池引流治疗重症原发性脑出血可以降低患者病死率低,提高患者生活质量。

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  • 亚低温治疗仪在颅内感染伴高热患者临床护理中的应用

    目的 探讨颅内感染伴高热患者应用亚低温治疗仪进行降温治疗的临床护理。 方法 回顾性分析2009年6月-2011年9月收治的89例颅内感染伴高热患者的临床资料,总结在降温治疗过程中,应用亚低温治疗仪的方法、疗效及护理观察。 结果 89例患者在抗感染治疗的同时使用亚低温治疗仪辅助降温治疗2~14 d,平均5.4 d。其中在4 h内降至设定温度者24例,在5~8 h降至设定温度者34例,在9~12 h降至设定温度者30例,1例隐球菌脑膜炎高热患者,因感染未得到有效控制,使用亚低温治疗仪后,体温下降不理想,病情无明显好转,患者自动出院。降温总有效率98.8%。 结论 颅内感染伴高热患者在抗感染治疗的基础上配合使用亚低温治疗仪治疗,能有效控制高热患者的体温,避免继发性颅内损伤及伴内环境紊乱,从而降低患者死亡率,提高护理质量,加快疾病的康复和防止并发症的发生。

    Release date:2016-09-07 02:37 Export PDF Favorites Scan
  • 脑干出血患者行血管内降温亚低温治疗护理一例

    Release date:2016-09-08 09:18 Export PDF Favorites Scan
  • 围生期脑卒中的研究进展及诊治现状

    围生期脑卒中是一种严重的神经系统疾病,近年来引起了临床医生的高度重视。由于该病缺乏特异性症状和体征,常漏诊和延误治疗,部分患者遗留严重的神经系统后遗症,影响患儿的生活质量。因此有必要提高对该病的认识,熟悉其病因和诊疗手段。现就围生期脑卒中的病因、相关危险因素及治疗的最新研究进展作一综述。

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  • Hypothermia Continuous Renal Replacement Therapy for Severe Heart Failure after Cardiac Surgery

    目的探讨亚低温联合持续肾脏替代治疗(CRRT)对心脏外科术后重症心力衰竭的临床效果 方法回顾性分析我中心2009年2月至2013年12月行心脏外科术后重症心力衰竭38例患者的临床资料,其中男18例、女20例,年龄55~74岁,虽应用大剂量血管活性药物及主动脉内球囊反搏(IABP)辅助循环,心力衰竭无改善,采用CRRT及亚低温联合治疗。监测患者在联合治疗前后心脏指数(CI)值、混合静脉血氧饱和度(SvO2)、尿量、肌酐(Cr)及乳酸(Lac)的变化。 结果亚低温联合CRRT治疗后,患者CI较治疗前明显改善[(2.3± 0.7)L/(min· m2)vs.(1.8± 0.2)L/(min· m2)],SvO2升高(62%± 5%vs.50%± 4%),乳酸明显降低[(8.6± 2.3)mmol/L vs.(3.0± 1.1)mmol/L],尿量明显增加[(2.5± 0.9)ml/h vs.(1.0± 0.7)ml/h],Cr明显下降[(140± 19)mmol/L vs.(292± 24)mmol/L]。 结论亚低温联合CRRT治疗心脏外科术后重症心力衰竭能有效改善循环功能,且操作简单易行。

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  • 巧用亚低温治疗仪治疗 29 周早产儿重度低体温复温的护理一例

    Release date:2017-02-22 03:47 Export PDF Favorites Scan
  • Extracorporeal membrane oxygenation combined with hypothermia therapy for children patients with refractory cardiac arrest after congenital heart disease surgery: A randomized controlled trial

    Objective To explore the value of extracorporeal membrane oxygenation(ECMO) combined with hypothermia therapy for children patients with refractory cardiac arrest after congenital heart disease surgery. Methods From January 2013 to June 2016, we conducted a prospective study of 23 children (18 males, 5 females at age of 7±11 months) who underwent ECMO for refractory cardiac arrest after congenital heart disease surgery. All patients were randomly divided into two groups: a standard group (11 patients) and a hypothermia group (12 patients). The patients of the standard group received standard therapy (the core body temperature maintaining at 37.0℃) and the hypothermia group received hypothermia therapy (the core body temperature maintaining at 33.0℃). The hospital discharge rate, the rate of weaning from ECMO and the morbidity were compared between the two groups. Results Eleven of 23 patients (47.8%) were weaned from ECMO successfully and 7 of 23 patients (30.4%) discharged from hospital. The hospital discharge rate between the hypothermia group (n=6, 50.0%) and the standard group (n=1, 9.1%) had no statistical difference (χ2=4.537, P=0.069). The rate of weaning from ECMO of the hypothermia group (n=9, 75.0%) was higher than that of the standard group (n=2, 18.2%, χ2=7.425, P=0.006). The morbidity between the two groups had no statistical difference. Conclusion Extracorporeal cardiopulmonary resuscitation can improve the survival rate of the children who suffered from refractory cardiac arrest after congenital heart disease surgery. There is no evidence that ECMO combined with hyperthermia therapy is better than the only ECMO in improving the discharge rate. But ECMO combined with hypothermia therapy has higher rate of weaning from ECMO than that of the only ECMO.

    Release date:2017-08-01 09:37 Export PDF Favorites Scan
  • Therapeutic effect of mild hypothermia on the inflammatory response and outcome in perioperative patients with acute Stanford type A aortic dissection: A randomized controlled trial

    Objective To explore the therapeutic effect of mild hypothermia on the inflammatory response, organ function and outcome in perioperative patients with acute Stanford type A aortic dissection (AAAD). Methods From February 2017 to February 2018, 56 patients with AAAD admitted in our department were enrolled and randomly allocated into two groups including a control group and an experimental group. After deep hypothermia circulatory arrest during operation, in the control group (n=28), the patients were rewarmed to normal body temperatures (36 to 37 centigrade degree), and which would be maintained for 24 hours after operation. While in the experimental group (n=28), the patients were rewarmed to mild hypothermia (34 to 35 centigrade degree), and the rest steps were the same to the control group. The thoracic drainage volume and the incidence of shivering at the first 24 hours after operation, inflammatory indicators and organ function during perioperation, and outcomes were compared between the two groups. There were 20 males and 8 females at age of 51.5±8.7 years in the control group, 24 males and 4 females at age of 53.3±11.2 years in the experimental group.Results There was no obvious difference in the basic information and operation information in patients between the two groups. Compared to the control group, at the 24th hour after operation, the level of peripheral blood matrix metalloproteinases (MMPs) was lower than that in the experimental group (P=0.008). In the experimental group, after operation, the awakening time was much shorter (P=0.008), the incidence of bloodstream infection was much lower (P=0.019). While the incidence of delirium, acute kidney injury (AKI), hepatic insufficiency, mechanical ventilation duration, intensive care unit (ICU) stays, or hospital mortality rate showed no statistical difference. And at the first 24 hours after operation, there was no difference in the thoracic drainage volume between the two groups, and no patient suffered from shivering. Conclusion The mild hypothermia therapy is able to shorten the awakening time and reduce the incidence of bloodstream infection after operation in the patients with AAAD, and does not cause the increase of thoracic drainage volume or shivering.

    Release date:2019-03-01 05:23 Export PDF Favorites Scan
  • The effects of esophageal cooling on lung injury and systemic inflammatory response after cardiopulmonary resuscitation in swine

    ObjectiveTo investigate the effects of esophageal cooling (EC) on lung injury and systemic inflammatory response after cardiopulmonary resuscitation in swine.MethodsThirty-two domestic male white pigs were randomly divided into sham group (S group, n=5), normothermia group (NT group, n=9), surface cooling group (SC group, n=9), and EC group (n=9). The animals in the S group only experienced the animal preparation. The animal model was established by 8 min of ventricular fibrillation and then 5 min of cardiopulmonary resuscitation in the other three groups. A normal temperature of (38.0±0.5)℃ was maintained by surface blanket throughout the experiment in the S and NT groups. At 5 min after resuscitation, therapeutic hypothermia was implemented via surface blanket or EC catheter to reach a target temperature of 33℃, and then maintained until 24 h post resuscitation, and followed by a rewarming rate of 1℃/h for 5 h in the SC and EC groups. At 1, 6, 12, 24 and 30 h after resuscitation, the values of extra-vascular lung water index (ELWI) and pulmonary vascular permeability index (PVPI) were measured, and meanwhile arterial blood samples were collected to measure the values of oxygenation index (OI) and venous blood samples were collected to measure the serum levels of tumor necrosis factor-α (TNF-α) and inerleukin-6 (IL-6). At 30 h after resuscitation, the animals were euthanized, and then the lung tissue contents of TNF-α, IL-6 and malondialdehyde, and the activities of superoxide dismutase (SOD) were detected.ResultsAfter resuscitation, the induction of hypothermia was significantly faster in the EC group than that in the SC group (2.8 vs. 1.5℃/h, P<0.05), and then its maintenance and rewarming were equally achieved in the two groups. The values of ELWI and PVPI significantly decreased and the values of OI significantly increased from 6 h after resuscitation in the EC group and from 12 h after resuscitation in the SC group compared with the NT group (all P<0.05). Additionally, the values of ELWI and PVPI were significantly lower and the values of OI were significantly higher from 12 h after resuscitation in the EC group than those in the SC group [ELWI: (13.4±3.1) vs. (16.8±2.7) mL/kg at 12 h, (12.4±3.0) vs. (16.0±3.6) mL/kg at 24 h, (11.1±2.4) vs. (13.9±1.9) mL/kg at 30 h; PVPI: 3.7±0.9 vs. 5.0±1.1 at 12 h, 3.4±0.8 vs. 4.6±1.0 at 24 h, 3.1±0.7 vs. 4.2±0.7 at 30 h; OI: (470±41) vs. (417±42) mm Hg (1 mm Hg=0.133 kPa) at 12 h, (462±39) vs. (407±36) mm Hg at 24 h, (438±60) vs. (380±33) mm Hg at 30 h; all P<0.05]. The serum levels of TNF-α and IL-6 significantly decreased from 6 h after resuscitation in the SC and EC groups compared with the NT group (all P<0.05). Additionally, the serum levels of IL-6 from 6 h after resuscitation and the serum levels of TNF-α from 12 h after resuscitation were significantly lower in the EC group than those in the SC group [IL-6: (299±23) vs. (329±30) pg/mL at 6 h, (336±35) vs. (375±30) pg/mL at 12 h, (297±29) vs. (339±36) pg/mL at 24 h, (255±20) vs. (297±33) pg/mL at 30 h; TNF-α: (519±46) vs. (572±49) pg/mL at 12 h, (477±77) vs. (570±64) pg/mL at 24 h, (436±49) vs. (509±51) pg/mL at 30 h; all P<0.05]. The contents of TNF-α, IL-6, and malondialdehyde significantly decreased and the activities of SOD significantly increased in the SC and EC groups compared with the NT group (all P<0.05). Additionally, lung inflammation and oxidative stress were further significantly alleviated in the EC group compared with the SC group [TNF-α: (557±155) vs. (782±154) pg/mg prot; IL-6: (616±134) vs. (868±143) pg/mg prot; malondialdehyde: (4.95±1.53) vs. (7.53±1.77) nmol/mg prot; SOD: (3.18±0.74) vs. (2.14±1.00) U/mg prot; all P<0.05].ConclusionTherapeutic hypothermia could be rapidly induced by EC after resuscitation, and further significantly alleviated post-resuscitation lung injury and systemic inflammatory response compared with conventional surface cooling.

    Release date:2019-12-12 04:12 Export PDF Favorites Scan
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